Li H, Zhou L-P, Luo J, Yu J-P, Yang H, Wei H-P
Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China; University of Chinese Academy of Sciences, Beijing, China.
Tuberculosis Reference Laboratory, Hubei Provincial Institute of Tuberculosis Control And Prevention, Wuhan, China.
Int J Tuberc Lung Dis. 2016 Apr;20(4):462-7. doi: 10.5588/ijtld.15.0745.
Colorimetric methods for detecting drug resistance in Mycobacterium tuberculosis are very attractive, as they are cheap, easy to use and require no costly apparatus. Although the current colorimetric methods have been used for other drugs, such as rifampicin and isoniazid, few of the colorimetric methods have been reported to have been used in performing drug susceptibility testing (DST) against pyrazinamide (PZA).
To develop a rapid and reliable colorimetric method for PZA DST using a monotetrazolium redox dye, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), as indicator of viability.
A total of 50 clinical isolates and three standard strains were tested using this colorimetric method and the BACTEC™ MGIT™ 960 system.
Initial test results showed that the PZA DST results were available in 4-6 days; the overall sensitivity and specificity of the CTC colorimetric method were respectively 97.1% and 81.3% in comparison with the MGIT 960 results.
These results suggest that the CTC colorimetric method is most rapid among the current PZA DST methods based on culture, and could be used for determining susceptibility to PZA of M. tuberculosis isolates.
用于检测结核分枝杆菌耐药性的比色法极具吸引力，因为它们成本低廉、易于使用且无需昂贵的仪器设备。尽管目前的比色法已用于检测其他药物，如利福平和异烟肼，但很少有比色法被报道用于对吡嗪酰胺（PZA）进行药物敏感性试验（DST）。
开发一种快速可靠的比色法用于PZA DST，使用单四氮唑氧化还原染料5-氰基-2,3-二甲基氯化四氮唑（CTC）作为活力指示剂。
使用这种比色法和BACTEC™ MGIT™ 960系统对50株临床分离株和3株标准菌株进行检测。
初步测试结果显示，PZA DST结果在4至6天内可得；与MGIT 960结果相比，CTC比色法的总体敏感性和特异性分别为97.1%和81.3%。
这些结果表明，在目前基于培养的PZA DST方法中，CTC比色法最为快速，可用于确定结核分枝杆菌分离株对PZA的敏感性。
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