Chen Juan, Liang Ru-Ping, Wu Lu-Lu, Qiu Jian-Ding
Department of Chemistry, Nanchang University, Nanchang, P. R. China.
Electrophoresis. 2016 Jul;37(12):1676-84. doi: 10.1002/elps.201600054. Epub 2016 Apr 13.
In this paper, using the self-polymerization of norepinephrine (NE) and its favorable film-forming property, a simple and green preparation approach was developed to modify a PDMS channel for enantioseparation of chiral compounds. After the PDMS microchip was filled with NE solution, poly(norepinephrine) (PNE) film was gradually formed and deposited on the inner wall of microchannel as permanent coating via the oxidation of NE by the oxygen dissolved in the solution. Due to possessing plentiful catechol and amine functional groups, the PNE-coated PDMS microchip exhibited much better wettability, more stable and suppressed EOF, and less nonspecific adsorption. The water contact angle and EOF of PNE-coated PDMS substrate were measured to be 13° and 1.68 × 10(-4) cm(2) V(-1) s(-1) , compared to those of 108° and 2.24 × 10(-4) cm(2) V(-1) s(-1) from the untreated one, respectively. Different kinds of chiral compounds, such as amino acid enantiomer, drug enantiomer, and peptide enantiomer were efficiently separated utilizing a separation length of 37 mm coupled with in-column amperometric detection on the PNE-coated PDMS microchips. This facile mussel-inspired PNE-based microchip system exhibited strong recognition ability, high-performance, admirable reproducibility, and stability, which may have potential use in the complex biological analysis.
在本文中，利用去甲肾上腺素（NE）的自聚合及其良好的成膜特性，开发了一种简单且绿色的制备方法来修饰聚二甲基硅氧烷（PDMS）通道，用于手性化合物的对映体分离。在PDMS微芯片中填充NE溶液后，聚去甲肾上腺素（PNE）膜通过溶液中溶解的氧气对NE的氧化作用逐渐形成并沉积在微通道内壁上，作为永久性涂层。由于含有大量的儿茶酚和胺官能团，涂有PNE的PDMS微芯片表现出更好的润湿性、更稳定且受抑制的电渗流（EOF）以及更少的非特异性吸附。涂有PNE的PDMS基底的水接触角和EOF分别测得为13°和1.68×10⁻⁴ cm² V⁻¹ s⁻¹，相比之下，未处理的基底分别为108°和2.24×10⁻⁴ cm² V⁻¹ s⁻¹。利用37 mm的分离长度，结合在涂有PNE的PDMS微芯片上进行柱内安培检测，能够有效分离不同种类的手性化合物，如氨基酸对映体、药物对映体和肽对映体。这种受贻贝启发的基于PNE的简便微芯片系统表现出强大的识别能力、高性能、令人钦佩的重现性和稳定性，在复杂生物分析中可能具有潜在应用。
