Buckingham Margaret
Department of Developmental and Stem Cell Biology, Institut Pasteur, Paris, France.
Curr Top Dev Biol. 2016;116:135-51. doi: 10.1016/bs.ctdb.2015.11.025. Epub 2016 Feb 17.
In this essay I trace my own research experience as a developmental biologist, from the study of cell differentiation in vitro to tissue formation and regeneration in vivo. Beginning with a thesis on histone modifications, I went on to study gene regulation during myogenesis, first in muscle cells in culture and then in the mouse embryo. Later, we also worked on muscle regeneration in the adult. Our work on striated muscle genes also led us into the field of cardiogenesis and characterization of cardiac progenitor cells that form the heart. Comments on the state of the art--changing concepts and the technological advances that underlie scientific progress--accompany this account, with concluding remarks about future directions.
在本文中，我追溯了自己作为一名发育生物学家的研究经历，从体外细胞分化研究到体内组织形成与再生研究。我最初的论文是关于组蛋白修饰的，之后继续研究肌发生过程中的基因调控，先是在培养的肌肉细胞中，然后是在小鼠胚胎中。后来，我们还开展了成体肌肉再生的研究。我们对横纹肌基因的研究也使我们进入了心脏发生领域，并对形成心脏的心脏祖细胞进行了表征。本文在讲述研究经历的同时，还对当前的技术水平——不断变化的概念以及作为科学进步基础的技术进展——进行了评论，并对未来方向作出了总结性评论。
