[巴西紫癜热。埃及嗜血杆菌侵袭性菌株的快速鉴定]

[Brazilian purpuric fever. Fast characterization of invasive strains of Haemophilus aegyptius].

作者信息

Brandileone M C, Vieira V S, Tondella M L, Sacchi C T, Landgraf I M, Zanella R C, Bibb W F, Irino K

出版信息

Rev Inst Med Trop Sao Paulo. 1989 Jul-Aug;31(4):221-7. doi: 10.1590/s0036-46651989000400003.

DOI:10.1590/s0036-46651989000400003

Abstract

Strains of H. aegyptius isolated during outbreak of Brazilian Purpuric Fever (BPF) in Brazil were characterized antigenically by slide agglutination test utilizing antiserum produced with a H. aegyptius strain isolated from blood culture from a patient with BPF. By means of this method, it were identified H. aegyptius strains responsible for outbreaks of conjunctivitis with identical antigenic characteristics to strains isolated from BPF. The sensitivity and specificity of slide seroagglutination test was 97.7% and 89.6% respectively; therefore this assay was efficient to be used as a screening method in the studies of purulent conjunctivitis for detecting high risk populations for BPF, and to implement measures that will increase the efficiency of epidemiologic surveillance.

摘要

在巴西爆发巴西紫癜热（BPF）期间分离出的埃及嗜血杆菌菌株，通过玻片凝集试验进行抗原特性鉴定，该试验使用由从一名BPF患者血培养物中分离出的埃及嗜血杆菌菌株产生的抗血清。通过这种方法，鉴定出了导致结膜炎暴发的埃及嗜血杆菌菌株，其抗原特性与从BPF中分离出的菌株相同。玻片血清凝集试验的敏感性和特异性分别为97.7%和89.6%；因此，该检测方法可有效地用作化脓性结膜炎研究中的筛查方法，以检测BPF的高危人群，并实施提高流行病学监测效率的措施。