Erwin A L, Munford R S
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas.
J Clin Microbiol. 1989 Apr;27(4):762-7. doi: 10.1128/jcm.27.4.762-767.1989.
Haemophilus influenzae biogroup aegyptius (H. aegyptius) has been identified as the etiologic agent of the recently described disease Brazilian purpuric fever (BPF). Although there is heterogeneity among the strains associated with conjunctivitis, isolates from patients with BPF appear to be derived from a single clone. The clinical presentation of BPF suggests that bacterial lipopolysaccharides (LPS) are involved in its pathogenesis. We prepared LPS from H. influenzae biogroup aegyptius and found them to be similar to H. influenzae type b LPS in apparent size (by sodium dodecyl sulfate-polyacrylamide gel electrophoresis), biological activities, and fatty acid composition. We compared LPS from BPF clone isolates with LPS from non-BPF clone isolates in tests of Limulus lysate activation, spleen cell mitogenesis, promotion of neutrophil adherence to LPS-treated endothelial cells, and the dermal Shwartzman reaction. In none of these activities were LPS from the BPF clone isolates more potent. Because LPS shed from growing bacteria may be involved in the pathogenesis of purpura, we also measured the rate at which LPS were released into culture medium during bacterial growth and found no significant difference between BPF clone and non-BPF clone isolates.
埃及嗜血杆菌生物群（埃及嗜血杆菌）已被确认为最近描述的巴西紫癜热（BPF）疾病的病原体。尽管与结膜炎相关的菌株存在异质性，但来自BPF患者的分离株似乎源自单一克隆。BPF的临床表现表明细菌脂多糖（LPS）参与了其发病机制。我们从埃及嗜血杆菌中制备了LPS，并发现它们在表观大小（通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳）、生物学活性和脂肪酸组成方面与b型流感嗜血杆菌LPS相似。我们在鲎试剂裂解物激活试验、脾细胞有丝分裂、中性粒细胞对LPS处理的内皮细胞的黏附促进以及皮肤施瓦茨曼反应试验中，比较了BPF克隆分离株的LPS与非BPF克隆分离株的LPS。在这些活性中，BPF克隆分离株的LPS均没有更强的效力。由于生长中的细菌释放的LPS可能参与紫癜的发病机制，我们还测量了细菌生长过程中LPS释放到培养基中的速率，发现BPF克隆和非BPF克隆分离株之间没有显著差异。
