Institute of Special Environmental Biophysics, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China.
Institute of Special Environmental Biophysics, Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, PR China.
Colloids Surf B Biointerfaces. 2016 Jun 1;142:325-333. doi: 10.1016/j.colsurfb.2016.03.002. Epub 2016 Mar 2.
A novel porous Fe/Fe-W alloy scaffold with a double-layer structured skeleton was prepared for the first time by electrodeposition. The microstructure of the scaffold was analysed by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and mercury porosimetry. Mechanical property, in vitro degradability and biocompatibility were tested by tensile test, immersion and a cytotoxicity test. The results showed that the scaffolds exhibited a cellular structure that is similar to that of cancellous bone and had a considerably large specific surface area. The skeleton of the scaffolds showed a double-layer structure that was composed of a hollow Fe skeleton wrapped in a thin layer of Fe-W alloy. The tensile strength and the apparent density are close to that of cancellous bone. It was also found that the different surface microstructures showed different effects on in vitro degradability and biocompatibility. In the immersion test, the corrosion rate decreased gradually as the immersion time increased. In the cytotoxicity test, the extraction medium of the pure Fe scaffold showed the lowest cell viability, followed by that of 1.5FeW as a close second. The extraction media of FeW, Fe1.5W and Fe2W were similar, and their cell viability was far above that of the Fe and 1.5FeW scaffolds. The structural style of the scaffolds presented in this paper is potentially useful and applicable to developing degradable scaffolds with a tailored corrosion rate.
首次通过电沉积制备了具有双层结构骨架的新型多孔 Fe/Fe-W 合金支架。通过 X 射线衍射（XRD）、扫描电子显微镜（SEM）、能谱（EDS）和压汞法分析了支架的微观结构。通过拉伸试验、浸泡和细胞毒性试验测试了机械性能、体外降解性和生物相容性。结果表明，支架呈现出类似于松质骨的细胞结构，具有相当大的比表面积。支架的骨架呈现出双层结构，由包裹在薄 Fe-W 合金层中的空心 Fe 骨架组成。拉伸强度和表观密度与松质骨相近。还发现不同的表面微观结构对体外降解性和生物相容性有不同的影响。在浸泡试验中，随着浸泡时间的增加，腐蚀速率逐渐降低。在细胞毒性试验中，纯 Fe 支架的提取介质显示出最低的细胞活力，其次是 1.5FeW。FeW、Fe1.5W 和 Fe2W 的提取介质相似，其细胞活力远高于 Fe 和 1.5FeW 支架。本文提出的支架结构样式具有潜在的有用性和适用性，可用于开发具有定制腐蚀速率的可降解支架。
