National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510641, China; Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Colloids Surf B Biointerfaces. 2016 Jun 1;142:344-350. doi: 10.1016/j.colsurfb.2016.03.015. Epub 2016 Mar 7.
In this article, the synthesis of a novel calcification-targeting nanoparticle (NP) is reported, which is realized through dopamine self-polymerization on the poly(lactic-co-glycolic acid) (PLGA) particle surface and subsequent alendronate conjugation. Cell viability and proliferation tests confirmed that such particle has low cytotoxicity and good biocompatibility. Experiments were designed to observe whether the synthesized NPs can pass through an obstructive hydrogel and directly bind themselves to hydroxyapatite (HA) NPs (mimicking calcified spots) and HA porous scaffolds (mimicking calcified tissues); and the result was positive, indicating ingenious targeting of NPs on calcifications. The calcification-targeting NPs are expected to be with promising applications on calcification-related disease diagnoses and therapies.
本文报道了一种新型钙化靶向纳米粒子（NP）的合成方法，该方法通过多巴胺在聚乳酸-共-羟基乙酸（PLGA）粒子表面的自聚合以及随后的阿仑膦酸钠接枝来实现。细胞活力和增殖试验证实，这种粒子具有低细胞毒性和良好的生物相容性。实验设计观察合成的 NPs 是否可以穿透阻塞性水凝胶并直接与羟基磷灰石（HA） NPs（模拟钙化点）和 HA 多孔支架（模拟钙化组织）结合；结果是肯定的，表明 NPs 对钙化的靶向作用巧妙。这种钙化靶向 NPs 有望在钙化相关疾病的诊断和治疗方面具有广阔的应用前景。
