EaStCHEM School of Chemistry, University of Edinburgh, David Brewster Road, Edinburgh, EH9 3FJ, UK.
The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK.
Sci Rep. 2018 Nov 26;8(1):17360. doi: 10.1038/s41598-018-35454-9.
A rapid and efficient method for the detection of hydroxyapatite (HAP) has been developed which shows superiority to existing well-established methods. This fluorescein-bisphosphonate probe is highly selective for HAP over other calcium minerals and is capable of detecting lower levels of calcification in cellular models than either hydrochloric acid-based calcium leaching assays or the Alizarin S stain. The probe has been shown to be effective in both in vitro vascular calcification models and in vitro bone calcification models. Moreover we have demonstrated binding of this probe to vascular calcification in rat aorta and to areas of microcalcification, in human vascular tissue, beyond the resolution of computed tomography in human atherosclerotic plaques. Fluorescein-BP is therefore a highly sensitive and specific imaging probe for the detection of vascular calcification, with the potential to improve not only ex vivo assessments of HAP deposition but also the detection of vascular microcalcification in humans.
已经开发出一种快速有效的检测羟磷灰石 (HAP) 的方法，该方法优于现有的成熟方法。这种荧光素双磷酸探针对 HAP 具有高度选择性，超过其他钙矿物质，并且能够在细胞模型中检测到比基于盐酸的钙浸出测定法或茜素 S 染色更低水平的钙化。该探针已被证明在体外血管钙化模型和体外骨钙化模型中均有效。此外，我们已经证明该探针能够与大鼠主动脉中的血管钙化以及人血管组织中的微钙化区域结合，其分辨率超过人动脉粥样硬化斑块中计算机断层扫描的分辨率。因此，荧光素-BP 是一种用于检测血管钙化的高灵敏度和特异性成像探针，不仅有可能改善 HAP 沉积的体外评估，而且有可能改善人血管微钙化的检测。
