Lin Qiang, De Vrieze Jo, Li Jiabao, Li Xiangzhen
Key Laboratory of Environmental and Applied Microbiology, CAS, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Laboratory of Microbial Ecology and Technology, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
Bioresour Technol. 2016 Jun;209:228-36. doi: 10.1016/j.biortech.2016.02.132. Epub 2016 Mar 3.
Temperature is a major factor determining the performance of the anaerobic digestion process. The microbial abundance, activity and interactional networks were investigated under a temperature gradient from 25°C to 55°C through amplicon sequencing, using 16S ribosomal RNA and 16S rRNA gene-based approaches. Comparative analysis of past accumulative elements presented by 16S rRNA gene-based analysis, and the in-situ conditions presented by 16S rRNA-based analysis, provided new insights concerning the identification of microbial functional roles and interactions. The daily methane production and total biogas production increased with temperature up to 50°C, but decreased at 55°C. Increased methanogenesis and hydrolysis at 50°C were main factors causing higher methane production which was also closely related with more well-defined methanogenic and/or related modules with comprehensive interactions and increased functional orderliness referred to more microorganisms participating in interactions. This research demonstrated the importance of evaluating functional roles and interactions of microbial community.
温度是决定厌氧消化过程性能的主要因素。通过基于16S核糖体RNA和16S rRNA基因的扩增子测序，在25°C至55°C的温度梯度下研究了微生物丰度、活性和相互作用网络。基于16S rRNA基因分析呈现的过去累积元素与基于16S rRNA分析呈现的原位条件的比较分析，为识别微生物功能作用和相互作用提供了新的见解。每日甲烷产量和总沼气产量随温度升高至50°C而增加，但在55°C时下降。50°C时产甲烷作用和水解作用的增强是导致更高甲烷产量的主要因素，这也与更明确的产甲烷和/或相关模块密切相关，这些模块具有全面的相互作用且功能有序性增加，意味着有更多微生物参与相互作用。本研究证明了评估微生物群落功能作用和相互作用的重要性。
