Jangra Ashok, Kwatra Mohit, Singh Tavleen, Pant Rajat, Kushwah Pawan, Sharma Yogita, Saroha Babita, Datusalia Ashok Kumar, Bezbaruah Babul Kumar
Department of Pharmacology and Toxicology, Laboratory of Neuroscience, National Institute of Pharmaceutical Education and Research, Guwahati, Assam, 781032, India.
Department of Biotechnology, University Institute of Engineering & Technology (UIET), Maharishi Dayanand University, Rohtak, Haryana, India.
Inflammation. 2016 Jun;39(3):1025-38. doi: 10.1007/s10753-016-0332-4.
The aim of the present study was to investigate the protective effects of curcumin alone and in combination with piperine against lipopolysaccharide (LPS)-induced neurobehavioral and neurochemical deficits in the mice hippocampus. Mice were treated with curcumin (100, 200, and 400 mg/kg, p.o.) and piperine (20 mg/kg, p.o.) for 7 days followed by LPS (0.83 mg/kg, i.p.) administration. Animals exhibited anxiety and depressive-like phenotype after 3 and 24 h of LPS exposure, respectively. LPS administration increased the oxido-nitrosative stress as evident by elevated levels of malondialdehyde, nitrite, and depletion of glutathione level in the hippocampus. Furthermore, we found raised level of pro-inflammatory cytokines (IL-1β and TNF-α) in the hippocampus of LPS-treated mice. Pretreatment with curcumin alleviated LPS-induced neurobehavioral and neurochemical deficits. Furthermore, co-administration of curcumin with piperine significantly potentiated the neuroprotective effect of curcumin. These results demonstrate that piperine enhanced the neuroprotective effect of curcumin against LPS-induced neurobehavioral and neurochemical deficits.
本研究的目的是调查姜黄素单独使用以及与胡椒碱联合使用对脂多糖（LPS）诱导的小鼠海马神经行为和神经化学缺陷的保护作用。小鼠分别用姜黄素（100、200和400mg/kg，口服）和胡椒碱（20mg/kg，口服）处理7天，随后腹腔注射LPS（0.83mg/kg）。LPS暴露3小时和24小时后，动物分别表现出焦虑和抑郁样表型。LPS给药增加了氧化亚硝化应激，海马中丙二醛、亚硝酸盐水平升高以及谷胱甘肽水平降低证明了这一点。此外，我们发现LPS处理的小鼠海马中促炎细胞因子（IL-1β和TNF-α）水平升高。姜黄素预处理减轻了LPS诱导的神经行为和神经化学缺陷。此外，姜黄素与胡椒碱联合给药显著增强了姜黄素的神经保护作用。这些结果表明，胡椒碱增强了姜黄素对LPS诱导的神经行为和神经化学缺陷的神经保护作用。
