Department of Zoology, MMV, Banaras Hindu University, Varanasi, 221005, India.
Inflammation. 2018 Oct;41(5):1922-1933. doi: 10.1007/s10753-018-0836-1.
Allergic asthma is an inflammatory condition accompanied by inflammation as well as oxidative stress. Supplementation of an anti-inflammatory agent having antioxidant properties may have therapeutic effects against this disease. Over the recent decades, the interest in combination therapy as new alternative medication has increased and it offers numerous benefits along with noticeable lack of toxicity as well as side effects. In this study, protective effects of curcumin alone and in combination with piperine were evaluated in mouse model of allergic asthma. Balb/c mice were sensitized on days 0, 7, and 14 and challenged from days 16-30 on alternate days with ovalbumin (OVA). Mice were pretreated with curcumin (Cur; 10 and 20 mg/kg) and piperine (Pip; 5 mg/kg) alone and in combination via the intraperitoneal route on days 16-30 and compared with intranasal curcumin (5 mg/kg) treatment. Blood, bronchoalveolar lavage fluid (BALF), and lungs were collected after mice were sacrificed on day 31st. Mice immunized with OVA have shown significant increase in airway inflammation and oxidative stress as determined by oxidative stress markers. A significant suppression was observed with all the treatments, but intranasal curcumin treatment group has shown maximum suppression. So, among all the treatment strategies utilized, intranasal curcumin administration was most appropriate in reducing inflammation and oxidative stress and possesses therapeutic potential against allergic asthma. Present study may prove the possibility of development of curcumin nasal drops towards treatment of allergic asthma.
变应性哮喘是一种炎症性疾病，伴有炎症和氧化应激。补充具有抗氧化特性的抗炎剂可能对这种疾病有治疗作用。在最近几十年，人们对联合治疗作为新的替代药物的兴趣增加了，它提供了许多好处，同时毒性和副作用也明显减少。在这项研究中，评估了姜黄素单独和与胡椒碱联合在过敏性哮喘小鼠模型中的保护作用。Balb/c 小鼠在第 0、7 和 14 天致敏，并在第 16-30 天每隔一天用卵清蛋白（OVA）进行攻毒。在第 16-30 天，通过腹腔途径预先用姜黄素（Cur；10 和 20mg/kg）和胡椒碱（Pip；5mg/kg）单独和联合处理小鼠，并与鼻内姜黄素（5mg/kg）处理进行比较。在第 31 天处死小鼠后收集血液、支气管肺泡灌洗液（BALF）和肺。用 OVA 免疫的小鼠表现出气道炎症和氧化应激的显著增加，这可以通过氧化应激标志物来确定。所有治疗都观察到显著的抑制作用，但鼻内姜黄素治疗组的抑制作用最大。因此，在所使用的所有治疗策略中，鼻内姜黄素给药最适合减轻炎症和氧化应激，并对过敏性哮喘具有治疗潜力。本研究可能证明开发姜黄素鼻滴剂治疗过敏性哮喘的可能性。
