Hirota Kikue, Aino Kenichi, Yumoto Isao
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo, Hokkaido 062-8517, Japan.
Laboratory of Environmental Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-8589, Japan.
Int J Syst Evol Microbiol. 2016 Jun;66(6):2247-2253. doi: 10.1099/ijsem.0.001015. Epub 2016 Mar 11.
Facultatively alkaliphilic strains, designated as strains IEB3T and IEB14, were isolated as indigo-reducing strains from a fermented Polygonum indigo (Polygonum tinctorium Lour) liquor sample prepared in our laboratory using a medium containing an indigo fermentation liquor as a sole substrate. The 16S rRNA gene sequence phylogeny and similarity suggested that strains IEB3T and IEB14 exhibit distinctive positions among the members of the genus Bacillus, and their closest neighbour was Bacillus nanhaiisediminis NH3T (similarity: 97.4 %) among the species with validly published names. The 16S rRNA sequence of strain IEB3Twas identical to that of strain IEB14. The cells of the isolates stained Gram-positive and were facultatively anaerobic, straight rods that were motile by a pair of subpolar flagella. Strains IEB3T and IEB14 grew at temperatures between 12 and 40 °C with optimum growth at 30‒33 °C and in the range of pH 7.5-12. Menaquinone-7 (MK-7) was detected as the major isoprenoid quinone. The DNA G+C contents of strains IEB3T and IEB14 were 39.0 and 39.1 mol%, respectively. The whole-cell fatty acid profile mainly (>10 %) consisted of iso-C14:0, iso-C15:0 and anteiso-C15:0. DNA-DNA hybridization revealed a low relatedness value between strain IEB3T and the phylogenetically most closely related species, Bacillus nanhaiisediminis JCM 16507T (<7 % ). On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, the isolates represent a novel species within a novel genus, for which the name Fermentibacillus polygoni gen. nov., sp. nov. is proposed. The type strain is IEB3T (=JCM 30817T=NCIMB 14984T).
兼性嗜碱菌株，命名为IEB3T和IEB14，是从我们实验室制备的以靛蓝发酵液为唯一底物的培养基培养的发酵蓼蓝（Polygonum tinctorium Lour）酒样中分离得到的靛蓝还原菌株。16S rRNA基因序列系统发育和相似性表明，IEB3T和IEB14菌株在芽孢杆菌属成员中处于独特位置，在有效发表名称的物种中，它们最接近的邻居是南海芽孢杆菌NH3T（相似性：97.4%）。IEB3T菌株的16S rRNA序列与IEB14菌株相同。分离株细胞革兰氏染色呈阳性，兼性厌氧，为直杆状，通过一对亚极鞭毛运动。IEB3T和IEB14菌株在12至40°C的温度下生长，最适生长温度为30-33°C，pH范围为7.5-12。检测到甲基萘醌-7（MK-7）为主要类异戊二烯醌。IEB3T和IEB14菌株的DNA G+C含量分别为39.0和39.1 mol%。全细胞脂肪酸谱主要（>10%）由异-C14:0、异-C15:0和anteiso-C15:0组成。DNA-DNA杂交显示IEB3T菌株与系统发育上最密切相关的物种南海芽孢杆菌JCM 16507T之间的相关性值较低（<7%）。基于表型、化学分类特征和系统发育数据，这些分离株代表了一个新属内的一个新物种，为此提出了新属名发酵芽孢杆菌属（Fermentibacillus），新种名蓼发酵芽孢杆菌（Fermentibacillus polygoni）。模式菌株为IEB3T（=JCM 30817T=NCIMB 14984T）。
Zotero 插件