Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
Laboratory of Environmental Microbiology, Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1437-1442. doi: 10.1099/ijs.0.034579-0. Epub 2012 Jul 27.
An indigo-reducing facultatively alkaliphilic and halophilic strain, designated strain A21(T), was isolated from a fermented Polygonum indigo (Polygonum tinctorium Lour.) liquor sample aged for 4 days prepared in a laboratory. 16S rRNA gene sequence phylogeny suggested that strain A21(T) was a member of the genus Oceanobacillus with the closest relative being the type strain of Oceanobacillus chironomi (similarity: 96.0 %). The cells of the isolate stained Gram-positive and were facultatively anaerobic straight rods that were motile by peritrichous flagella. The strain grew between 18 and 48 °C with optimum growth at 39 °C. It grew in the pH range of 7-12. It hydrolysed casein, gelatin and Tween 20 but not Tweens 40, 60 and 80, starch or DNA. No isoprenoid quinone was detected and the DNA G+C content was 39.7 mol%. The whole-cell fatty acid profile mainly consisted of iso-C15 : 0, anteiso-C15 : 0 and C16 : 0. DNA-DNA hybridization experiments with O. chironomi revealed 13 % relatedness. Owing to the differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analyses based on 16S rRNA gene sequences and DNA-DNA relatedness data from reported Oceanobacillus species, the isolate merits classification as a representative of a novel species, for which the name Oceanobacillus indicireducens sp. nov. is proposed. The type strain is A21(T) ( = JCM 17251(T) = NCIMB 14685(T)). The description of the genus Oceanobacillus is also emended.
一株可还原靛蓝的兼性嗜碱和嗜盐菌株，命名为 A21(T)，是从在实验室中陈酿 4 天的发酵的蓼蓝（Polygonum tinctorium Lour.）酒样中分离得到的。16S rRNA 基因序列系统发育分析表明，菌株 A21(T) 是海洋杆菌属的一个成员，与模式菌株海洋杆菌（Chironomus）最接近（相似性：96.0%）。该分离株的细胞染色呈革兰氏阳性，为兼性厌氧菌直杆状，通过周生鞭毛运动。该菌株在 18-48°C 之间生长，最适生长温度为 39°C。它在 pH 值为 7-12 的范围内生长。它水解酪蛋白、明胶和吐温 20，但不水解吐温 40、60 和 80、淀粉或 DNA。未检测到异戊烯醌，DNA G+C 含量为 39.7 mol%。全细胞脂肪酸谱主要由 iso-C15:0、anteiso-C15:0 和 C16:0 组成。与海洋杆菌（Chironomus）的 DNA-DNA 杂交实验显示 13%的相关性。由于表型和化学分类特征的差异，以及基于 16S rRNA 基因序列和来自已报道的海洋杆菌属物种的 DNA-DNA 相关性数据的系统发育分析，该分离株值得分类为一个新种的代表，因此建议使用 Oceanobacillus indicireducens sp. nov. 这个名称。模式菌株为 A21(T)（=JCM 17251(T)=NCIMB 14685(T)）。海洋杆菌属的描述也得到了修订。
