Hirota Kikue, Nishita Masatoshi, Tu Zhihao, Matsuyama Hidetoshi, Yumoto Isao
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8571, Japan.
School of Biological Science and Engineering, Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1123-1129. doi: 10.1099/ijsem.0.002636. Epub 2018 Feb 16.
The indigo-reducing, facultatively anaerobic and obligately alkaliphilic strains Bf-1, Bf-2 and Bf-4 were isolated from an indigo fermentation liquor used for dyeing, which uses sukumo [composted Polygonum indigo (Polygonum tinctorium Lour.) leaves] as a basic ingredient and was obtained from a craft centre in Date City, Hokkaido, Japan. The 16S rRNA gene sequence analyses indicated that the closest neighbours of strain Bf-1 are Bacillus maritimus DSM 100413 (98.3 % 16S rRNA gene sequence similarity), Bacillus persicus DSM 25386 (98.2 %) and Bacillus rigiliprofundi LMG 28275 (97.7 %). The 16S rRNA gene sequence of strain Bf-1 was almost identical to the sequences of strains Bf-2 and Bf-4 (99.9 %). Cells of strain Bf-1 stained Gram-positive and formed straight rods that achieved motility through a pair of subpolar flagella. Strain Bf-1 grew at temperatures of between 15 and 45 °C with optimum growth at 33‒40 °C. The strain grew in the pH range of pH 8‒12, with optimum growth at pH 10. The isoprenoid quinone detected was menaquinone-7 (MK-7), and the DNA G+C content was 41.7 %. The whole-cell fatty acid profile mainly (>10 %) consisted of iso-C15 : 0 and iso-C16 : 0. Phylogenetically related neighbours, although demonstrating high 16S rRNA gene sequence similarity (>97.6 %) with strain Bf-1, exhibited less than 9 % relatedness in DNA-DNA hybridization experiments. Based on evidence from this polyphasic study, the isolates represent a novel species, for which the name Bacillus fermenti sp. nov. is proposed. The type strain of this species is Bf-1 (=JCM 31807=NCIMB 15079).
从用于染色的靛蓝发酵液中分离出了具有靛蓝还原能力、兼性厌氧且专性嗜碱的菌株Bf-1、Bf-2和Bf-4，该发酵液以蓼蓝（蓼蓝叶）堆肥作为基本成分，取自日本北海道伊达市的一个工艺中心。16S rRNA基因序列分析表明，菌株Bf-1的最相近亲缘种是海芽孢杆菌DSM 100413（16S rRNA基因序列相似性为98.3%）、波斯芽孢杆菌DSM 25386（98.2%）和深岸芽孢杆菌LMG 28275（97.7%）。菌株Bf-1的16S rRNA基因序列与菌株Bf-2和Bf-4的序列几乎相同（99.9%）。菌株Bf-1的细胞革兰氏染色呈阳性，形成直杆状，通过一对亚极鞭毛实现运动。菌株Bf-1在15至45 °C的温度下生长，最适生长温度为33至40 °C。该菌株在pH 8至12的范围内生长，最适生长pH为10。检测到的类异戊二烯醌为甲基萘醌-7（MK-7），DNA G+C含量为41.7%。全细胞脂肪酸谱主要（>10%）由异-C15:0和异-C16:0组成。尽管在系统发育上相关的亲缘种与菌株Bf-1的16S rRNA基因序列相似性较高（>97.6%），但在DNA-DNA杂交实验中显示出的相关性低于9%。基于这项多相研究的证据，这些分离株代表一个新物种，为此提议将其命名为发酵芽孢杆菌（Bacillus fermenti sp. nov.）。该物种的模式菌株是Bf-1（=JCM 31807=NCIMB 15079）。
