Fried Eric S, Luchan Joshua, Gilchrist M Lane
Department of Chemical Engineering and ‡Department of Biomedical Engineering, The City College of the City University of New York , 140th Street and Convent Avenue, New York, New York 10031, United States.
Langmuir. 2016 Apr 12;32(14):3470-5. doi: 10.1021/acs.langmuir.6b00008. Epub 2016 Mar 28.
Supported lipid bilayers (SLBs) are ideally suited for the study of biomembrane-biomembrane interactions and for the biomimicry of cell-to-cell communication, allowing for surface ligand displays that contain laterally mobile elements. However, the SLB paradigm does not include three-dimensionality and biocompatibility. As a way to bypass these limitations, we have developed a biodegradable form of microsphere SLBs, also known as proteolipobeads (PLBs), using PLGA microspheres. Microspheres were synthesized using solvent evaporation and size selected with fluorescence activated cell sorting (FACS). Biomembranes were covalently tethered upon fusion to microsphere supports via short-chain PEG spacers connecting membrane-integrated α-helical peptides and the microsphere surface, affecting membrane diffusivity and mobility as indicated by confocal FRAP analysis. Membrane heterogeneities, which are attributed to PLGA hydrophobicity and rough surface topography, are curtailed by the addition of PEG tethers. This method allows for the presentation of tethered, laterally mobile biomembranes in three dimensions with functionally embedded attachment peptides for mobile ligand displays.
支撑脂质双层（SLB）非常适合用于研究生物膜 - 生物膜相互作用以及细胞间通讯的仿生学，它能够实现包含横向移动元件的表面配体展示。然而，SLB模式并不具备三维性和生物相容性。作为克服这些限制的一种方法，我们利用聚乳酸 - 羟基乙酸共聚物（PLGA）微球开发了一种可生物降解形式的微球SLB，也称为蛋白脂质珠（PLB）。微球通过溶剂蒸发法合成，并使用荧光激活细胞分选（FACS）进行大小筛选。生物膜通过连接膜整合α - 螺旋肽和微球表面的短链聚乙二醇（PEG）间隔物在与微球载体融合时共价连接，共聚焦荧光恢复后漂白（FRAP）分析表明这会影响膜的扩散率和流动性。归因于PLGA疏水性和粗糙表面形貌的膜异质性通过添加PEG连接物得以减少。这种方法能够在三维空间中呈现带有用于移动配体展示的功能性嵌入附着肽的拴系、横向移动生物膜。
