Sezgin Erdinc, Schwille Petra
Biophysics/BIOTEC, TU Dresden, Tatzberg, Dresden, Germany.
Mol Membr Biol. 2012 Aug;29(5):144-54. doi: 10.3109/09687688.2012.700490. Epub 2012 Jul 26.
Constituting functional interactions between proteins and lipid membranes is one of the essential features of cellular membranes. The major challenge of quantitatively studying these interactions in living cells is the multitude of involved components that are difficult, if not impossible, to simultaneously control. Therefore, there is great need for simplified but still sufficiently detailed model systems to investigate the key constituents of biological processes. To specifically focus on interactions between membrane proteins and lipids, several membrane models have been introduced which recapitulate to varying degrees the complexity and physicochemical nature of biological membranes. Here, we summarize the presently most widely used minimal model membrane systems, namely Supported Lipid Bilayers (SLBs), Giant Unilamellar Vesicles (GUVs) and Giant Plasma Membrane Vesicles (GPMVs) and their applications for protein-membrane interactions.
构成蛋白质与脂质膜之间的功能相互作用是细胞膜的基本特征之一。在活细胞中定量研究这些相互作用的主要挑战在于，众多参与成分即便并非完全不可能同时控制，也是很难做到的。因此，迫切需要简化但仍足够详细的模型系统来研究生物过程的关键组成部分。为了特别关注膜蛋白与脂质之间的相互作用，人们引入了几种膜模型，这些模型不同程度地概括了生物膜的复杂性和物理化学性质。在此，我们总结了目前使用最广泛的最小模型膜系统，即支撑脂质双层（SLB）、巨型单层囊泡（GUV）和巨型质膜囊泡（GPMV）及其在蛋白质-膜相互作用中的应用。
