Montagner Anelise F, Opdam Niek J M, Ruben Jan L, Bronkhorst Ewald M, Cenci Maximiliano S, Huysmans Marie-Charlotte D N J M
School of Dentistry, Department of Restorative Dentistry, Federal University of Pelotas, Gonçalves Chaves 457, 96015-560 Pelotas, RS, Brazil; College of Dental Sciences, Department of Preventive and Restorative Dentistry, Radboud University Medical Center, Philips van Leydenlaan 25, 6525 EX Nijmegen, The Netherlands.
College of Dental Sciences, Department of Preventive and Restorative Dentistry, Radboud University Medical Center, Philips van Leydenlaan 25, 6525 EX Nijmegen, The Netherlands.
Dent Mater. 2016 May;32(5):668-75. doi: 10.1016/j.dental.2016.02.005. Epub 2016 Mar 10.
This in vitro study aimed to compare dentin wall caries development at different composite-dentin interfaces.
Dentin samples (10.4 mm(2)) were restored with composite resin using two adhesive systems (etch-and-rinse and self-etch techniques). Different composite-dentin interfaces with gaps were produced: (a) failed bonded, which were fractured at interface after being submitted to aging protocols (no aging, mechanical loading or water storage); (b) non-bonded interfaces, both without any adhesive material or with adhesive material applied only on the dentin. Adhesively fractured and non-bonded samples were subjected to a lactic acid gel (pH=5) caries model with a continuous opening/closing movement of the interfacial gap for 10 days. Transverse wavelength-independent microradiographs were taken, and lesion depth and mineral loss were measured. Data were analyzed with linear mixed-effects regression models.
Caries development differed among the composite-dentin interfaces (p<0.001). The non-bonded interface with adhesive material on the dentin showed less lesion depth than the failed bonded groups, while the non-bonded interface without adhesive on dentin showed the deepest wall lesions. Difference between the adhesive systems was observed only in the non-bonded groups (p=0.003), with the self-etch adhesive applied on the dentin showing more severe lesions. Samples broken after mechanical loading aging showed deeper lesions than those broken after water storage (p<0.001).
Composite-dentin interfaces failed after aging presented different demineralization from interfaces that were never bonded, indicating that the restorative treatment changes the tissue in a way relevant to secondary caries development.
本体外研究旨在比较不同复合树脂 - 牙本质界面处牙本质壁龋的发展情况。
使用两种粘结系统（酸蚀冲洗和自酸蚀技术）用复合树脂修复牙本质样本（10.4平方毫米）。制备出具有间隙的不同复合树脂 - 牙本质界面：（a）粘结失败组，在经过老化处理（未老化、机械加载或水储存）后在界面处断裂；（b）非粘结界面，既无任何粘结材料，或仅在牙本质上应用了粘结材料。对粘结断裂和非粘结样本采用乳酸凝胶（pH = 5）龋模型，界面间隙进行连续开闭运动10天。拍摄横向与波长无关的显微放射照片，并测量病变深度和矿物质损失。数据采用线性混合效应回归模型进行分析。
复合树脂 - 牙本质界面之间的龋发展情况存在差异（p < 0.001）。牙本质上有粘结材料的非粘结界面的病变深度小于粘结失败组，而牙本质上无粘结剂的非粘结界面的壁部病变最深。仅在非粘结组中观察到粘结系统之间存在差异（p = 0.003），牙本质上应用自酸蚀粘结剂的病变更严重。机械加载老化后断裂的样本比水储存后断裂的样本病变更深（p < 0.001）。
老化后失败的复合树脂 - 牙本质界面与从未粘结的界面呈现出不同的脱矿情况，表明修复治疗以与继发龋发展相关的方式改变了组织。
