Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil; Radboud University Medical Center, Radboud Institute for Health Sciences, Department of Dentistry, Nijmegen, The Netherlands.
Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, RS, Brazil.
J Dent. 2016 Sep;52:63-9. doi: 10.1016/j.jdent.2016.07.007. Epub 2016 Jul 18.
This study aimed to evaluate the dentin wall carious lesion development of different composite-dentin interfaces in the presence of two adhesive bonding materials in the gaps, using a microcosm biofilm model.
Dentin samples were prepared (10.4mm(2)) and restored with a composite resin using two adhesive systems (etch-and-rinse and self-etch techniques). Different conditions with respect to composite-dentin interfaces were produced with a 200μm gap: failed bonded without ageing or after mechanical ageing, or non-bonded with or without the presence of adhesive material on the dentin wall. For cariogenic challenge, specimens were subjected to a biofilm microcosm model for 14days to create caries-like wall lesions. Before and after caries development, transverse wavelength-independent microradiography images were taken, and lesion depth and mineral loss were measured. Data were analysed with linear regression models (p<0.05).
The composite-dentin interface conditions significant influenced the caries development: lesion development was reduced by the presence of the adhesive material on dentin wall, while lesion development was increased by the mechanical ageing (p=0.019). There was no difference between the adhesive materials (p values>0.05).
Different composite-dentin interfaces influence wall lesion development in gaps, with the interfaces submitted to ageing showing less carious protection than those interfaces with the presence of adhesive covering the dentin.
The presence of adhesive bonding material in the gaps plays a role on the wall caries lesion development.
本研究旨在使用微宇宙生物膜模型评估两种不同的牙本质-复合树脂粘结界面在存在间隙中两种粘结材料时的牙本质壁龋损的发展情况。
制备牙本质样本（10.4mm²）并用复合树脂通过两种粘结系统（酸蚀-冲洗和自酸蚀技术）进行修复。在 200μm 的间隙处产生了不同的牙本质-复合树脂界面条件：未老化或机械老化后的粘结失败，或无粘结但在牙本质壁上存在粘结材料。为了进行致龋挑战，将标本置于生物膜微宇宙模型中 14 天以产生类似龋的壁损。在龋损发展前后，拍摄了横向波长独立的显微放射图像，并测量了病变深度和矿物质损失。使用线性回归模型进行数据分析（p<0.05）。
牙本质-复合树脂界面条件显著影响龋损的发展：牙本质壁上粘结材料的存在减少了龋损的发展，而机械老化增加了龋损的发展（p=0.019）。两种粘结材料之间没有差异（p 值>0.05）。
不同的牙本质-复合树脂界面在间隙中影响壁损的发展，经过老化处理的界面比有粘结材料覆盖的界面的龋病保护作用更小。
间隙中粘结材料的存在对壁龋损的发展起着作用。
