Waddell A W, Bird C C, Currie A R
Br J Cancer. 1977 Aug;36(2):187-91. doi: 10.1038/bjc.1977.176.
Concentrations of methylprednisolone which have lethal effects on human lymphoblastoid cell lines in vitro cause a reduction both in the uptake of uridine and thymidine into acid-soluble material and their incorporation into acid-insoluble material. These effects are virtually instantaneous, which indicates that they do not depend on alterations in gene activity. Normal uptake of nucleosides into cells is by both simple and facilitated diffusion, and methylprednisolone appears to act directly on the cell surface to inhibit only facilitated diffusion uptake.
在体外对人淋巴母细胞系有致死作用的甲泼尼龙浓度,会使尿苷和胸苷摄取到酸溶性物质中的量减少,以及它们掺入酸不溶性物质中的量减少。这些作用几乎是瞬间发生的,这表明它们不依赖于基因活性的改变。核苷正常进入细胞是通过简单扩散和易化扩散,而甲泼尼龙似乎直接作用于细胞表面,仅抑制易化扩散摄取。