Najib Abdellaoui, Kim Min Sun, Choi Seung Hyuk, Kang Yue Jai, Kim Ki Hong
Department of Aquatic Life Medicine, Pukyong National University, Busan, 608-737, South Korea.
Department of Aquatic Life and Medical Sciences, Sun Moon University, Asan-si, Chungnam, 336-708, South Korea.
Fish Shellfish Immunol. 2016 Apr;51:384-391. doi: 10.1016/j.fsi.2016.03.014. Epub 2016 Mar 11.
To know the effect of viral hemorrhagic septicemia virus (VHSV) infection on the cellular microRNA expression profile in olive flounder (Paralichthys olivaceus), fish were infected with VHSV, and cellular microRNAs expression was analyzed at 0 (control), 6, 12, 24, 48 and 72 h post-infection (h.p.i.) by the high-throughput sequencing. A total of 372 mature miRNAs were identified, and, among them, 63 miRNAs were differentially expressed during VHSV infection. The differentially expressed microRNAs number was greatly increased from 24 h.p.i. compared to the number at 6 and 12 h.p.i., suggesting that the alteration of microRNAs expression by VHSV infection may be related to the progression of VHSV disease. The target prediction analysis, the GO enrichment analysis, and the KEGG pathway analysis of the predicted target genes showed that various biological pathways could be affected by VHSV infection through the down-regulation or up-regulation of host miRNAs. The present results provide a basic information on the microRNAs related to VHSV infection in olive flounder. Considering broad effects of microRNAs on various biological pathways, data in this study can be used to interpret the mechanism of VHSV pathogenesis, which, vice versa, can be used to develop control measures against VHSV.
为了解病毒性出血性败血症病毒（VHSV）感染对牙鲆（Paralichthys olivaceus）细胞微小RNA表达谱的影响，将牙鲆感染VHSV，并在感染后0（对照）、6、12、24、48和72小时（h.p.i.）通过高通量测序分析细胞微小RNA的表达。共鉴定出372个成熟的微小RNA，其中63个微小RNA在VHSV感染期间差异表达。与6和12 h.p.i.时相比，差异表达的微小RNA数量从24 h.p.i.开始大幅增加，这表明VHSV感染引起的微小RNA表达变化可能与VHSV疾病的进展有关。对预测的靶基因进行靶标预测分析、GO富集分析和KEGG通路分析表明，VHSV感染可通过下调或上调宿主微小RNA影响多种生物学途径。本研究结果提供了与牙鲆VHSV感染相关的微小RNA的基础信息。考虑到微小RNA对多种生物学途径的广泛影响，本研究数据可用于解释VHSV发病机制，反之，也可用于制定针对VHSV的防控措施。
