[内脏高敏感性幼鼠脊髓背角中脑源性神经营养因子的表达谱]
[Expression profiles of brain-derived neurotrophic factor in the spinal dorsal horn of young rats with visceral hypersensitivity].
作者信息
Wu Bin, Xu Chun, Huang Huan-Huan
机构信息
Department of Pediatrics, First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China.
出版信息
Zhongguo Dang Dai Er Ke Za Zhi. 2016 Mar;18(3):277-81. doi: 10.7499/j.issn.1008-8830.2016.03.017.
OBJECTIVE
To explore the relationship between the expression of brain-derived neurotrophic factor (BDNF) in the spinal dorsal horn and the increase in visceral hypersensitivity in young rats by establishing a young rat model of visceral hypersensitivity by neonatal maternal separation (NMS).
METHODS
Thirty-two newborn Sprague-Dawley rats were randomly and equally divided into four groups by a 2×2 factorial design: control, NMS, colorectal distension (CRD), and NMS+CRD. The newborn rats in the NMS and NMS+CRD groups were subjected to 3-hour daily maternal separation from days 2 to 14 after birth to establish a model of visceral hypersensitivity, while the rats in the control and CRD groups received no treatment after birth. At 6 weeks after birth, the CRD and CRD+NMS groups received CRD stimulation. The streptavidin-biotin complex immunohistochemical method was used to determine the expression of BDNF in the spinal dorsal horn. The immunohistochemical score (IHS) was calculated based on the percentage of BDNF-positive cells and color intensity. The percentage of BDNF-positive cells in the spinal dorsal horn and IHS were analyzed by factorial analysis of variance.
RESULTS
The expression of BDNF was detected bilaterally in the spinal dorsal horn at different levels in the four groups. The percentage of BDNF-positive cells and IHS were significantly higher in the NMS and NMS+CRD groups than in the control group (P<0.05). The results of factorial analysis of variance indicated that NMS significantly increased the percentage of BDNF-positive cells in the spinal dorsal horn and IHS; a single CRD stimulation had no effects on the IHS of BDNF-positive cells in the spinal dorsal horn; there was no interaction between NMS and a single CRD stimulation.
CONCLUSIONS
The over-expression of BDNF in the spinal dorsal horn may be involved in high visceral hypersensitivity in young rats induce by NMS.
目的
通过新生母鼠分离（NMS）建立幼鼠内脏高敏模型，探讨脊髓背角脑源性神经营养因子（BDNF）表达与幼鼠内脏高敏性增加之间的关系。
方法
采用2×2析因设计将32只新生Sprague-Dawley大鼠随机等分为四组：对照组、NMS组、结直肠扩张（CRD）组和NMS+CRD组。NMS组和NMS+CRD组新生大鼠在出生后第2天至第14天每天进行3小时母鼠分离，以建立内脏高敏模型，而对照组和CRD组大鼠出生后未接受处理。出生6周后，CRD组和CRD+NMS组接受CRD刺激。采用链霉亲和素-生物素复合物免疫组织化学方法测定脊髓背角BDNF的表达。根据BDNF阳性细胞百分比和显色强度计算免疫组织化学评分（IHS）。采用析因方差分析脊髓背角BDNF阳性细胞百分比和IHS。
结果
四组不同节段脊髓背角均双侧检测到BDNF表达。NMS组和NMS+CRD组BDNF阳性细胞百分比和IHS均显著高于对照组（P<0.0５）。析因方差分析结果表明，NMS显著增加脊髓背角BDNF阳性细胞百分比和IHS；单次CRD刺激对脊髓背角BDNF阳性细胞IHS无影响；NMS与单次CRD刺激之间无交互作用。
结论
脊髓背角BDNF过表达可能参与NMS诱导的幼鼠高内脏敏感性。
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