Giorgakis Emmanouil, Ramesh Bala, Kamali-Dashtarzheneh Ashkan, Fusai Giuseppe Kito, Imber Charles, Tsironis Dimitrios, Loizidou Marilena
Division of Surgery and Interventional Science, Royal Free Campus, University College London, London, UK Institute of Liver Studies, King's College Hospital, London, UK
Division of Surgery and Interventional Science, Royal Free Campus, University College London, London, UK.
Anticancer Res. 2016 Mar;36(3):861-7.
There is dire need for discovery of novel pancreatic cancer biomarkers and of agents with the potential for simultaneous diagnostic and therapeutic capacity. This study demonstrates calreticulin expression on hamster pancreatic adenocarcinoma via bio-conjugated gold quantum dots (AuQDs).
Hamster pancreatic adenocarcinoma cells were cultured, fixed and incubated with fluorescent AuQDs, bio-conjugated to anti-calreticulin antibodies. Anti-calreticulin and AuQDs were produced in-house. AuQDs were manufactured to emit in the near-infrared. Cells were further characterized under confocal fluorescence.
AuQDs were confirmed to emit in the near-infrared. AuQD bio-conjugation to calreticulin was confirmed via dot-blotting. Upon laser excitation and post-incubation with bio-conjugated AuQDs, pancreatic cancer cell lines emitted fluorescence in near-infrared.
Hamster pancreatic cancer cells express calreticulin, which may be labelled with AuQDs. This study demonstrates the application of nanoparticle-based theranostics in pancreatic cancer. Such biomarker-targeting nanosystems are anticipated to play a significant role in the management of pancreatic cancer.
迫切需要发现新型胰腺癌生物标志物以及具有同时诊断和治疗能力潜力的药物。本研究通过生物共轭金量子点（AuQDs）证明了钙网蛋白在仓鼠胰腺腺癌上的表达。
培养仓鼠胰腺腺癌细胞，固定后与生物共轭抗钙网蛋白抗体的荧光AuQDs孵育。抗钙网蛋白和AuQDs均为自制。AuQDs被制造为在近红外区域发射。在共聚焦荧光下对细胞进行进一步表征。
证实AuQDs在近红外区域发射。通过斑点印迹法证实了AuQDs与钙网蛋白的生物共轭。在用生物共轭AuQDs进行激光激发和孵育后，胰腺癌细胞系在近红外区域发出荧光。
仓鼠胰腺癌细胞表达钙网蛋白，其可用AuQDs标记。本研究证明了基于纳米颗粒的诊疗一体化在胰腺癌中的应用。这种靶向生物标志物的纳米系统有望在胰腺癌的管理中发挥重要作用。
