利用荧光金纳米颗粒研究结直肠癌中的机械生长因子表达
Mechano-growth Factor Expression in Colorectal Cancer Investigated With Fluorescent Gold Nanoparticles.
作者信息
Alagaratnam Swethan, Yang Shi-Yu, Loizidou Marilena, Fuller Barry, Ramesh Bala
机构信息
University College London Centre for Nanotechnology and Regenerative Medicine, Division of Surgery and Interventional Science, University College London, Royal Free Campus, London, U.K.
University Department of Surgery and Liver Transplant Unit, University College London, Royal Free Campus, London, U.K.
出版信息
Anticancer Res. 2019 Apr;39(4):1705-1710. doi: 10.21873/anticanres.13276.
BACKGROUND/AIM: Fluorescent gold nanoparticles demonstrate strong photoluminescence, photostability, and low cellular toxicity, making them attractive agents for biomedical applications. Mechano-growth factor (MGF) is an isoform of IGF1 and its expression has been demonstrated in malignancies including prostate cancer.
MATERIALS AND METHODS
Near-infrared-emitting gold nanoparticles (AuNPs) were synthesized and conjugated to MGF. Following characterization and confirmation of conjugation, these AuNPs were used to investigate the expression of MGF in colon cancer cell lines (HT29 and SW620) and tissues comparing normal and colon cancer. The prostate cancer cell line PC3 and adenocarcinoma tissues were used as positive controls.
RESULTS
Colon cancer cell lines, adenocarcinoma tissues and polyp tissues demonstrated evidence of MGF peptide expression, which was not found in normal colon tissues and human umbilical vein endothelial cells.
CONCLUSION
MGF appears to be overexpressed in colon cancer tissues, offering a potential unique target for imaging and drug delivery in colon cancer.
背景/目的：荧光金纳米颗粒具有强的光致发光、光稳定性和低细胞毒性，使其成为生物医学应用中有吸引力的试剂。机械生长因子（MGF）是IGF1的一种异构体，其表达已在包括前列腺癌在内的恶性肿瘤中得到证实。
材料与方法
合成了近红外发射金纳米颗粒（AuNPs）并将其与MGF偶联。在对偶联进行表征和确认后，这些AuNPs用于研究MGF在结肠癌细胞系（HT29和SW620）以及比较正常结肠组织和结肠癌组织中的表达。前列腺癌细胞系PC3和腺癌组织用作阳性对照。
结果
结肠癌细胞系、腺癌组织和息肉组织显示出MGF肽表达的证据，而在正常结肠组织和人脐静脉内皮细胞中未发现。
结论
MGF似乎在结肠癌组织中过表达，为结肠癌的成像和药物递送提供了潜在的独特靶点。
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