锌（II）与2-氨基乙硫醇盐酸盐配体的合成、晶体结构、光谱性质及与小牛胸腺DNA的相互作用

Synthesis, Crystal Structure, Spectroscopic Properties, and Interaction with Ct-DNA of Zn(II) with 2-Aminoethanethiol Hydrochloride Ligand.

作者信息

Shu Xu-Gang, Wu Chun-Li, Li Cui-Jin, Zhang Min, Wan Ke, Wu Xin

机构信息

College of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

College of Light Industry and Chemical Engineering, Guangdong University of Technology, Guangzhou 510006, China.

出版信息

Bioinorg Chem Appl. 2016;2016:2691253. doi: 10.1155/2016/2691253. Epub 2016 Feb 10.

The zinc(II) complex (C2H6NS)2Zn·ZnCl2 was synthesized with 2-aminoethanethiol hydrochloride and zinc sulfate heptahydrate as the raw materials in aqueous solution. The composition and structure of the complex were characterized by elemental analysis, infrared spectra, single crystal X-ray diffraction, and thermogravimetry. The crystal structure of the zinc(II) complex belongs to monoclinic system, space group P 21/n, with cell parameters of a = 0.84294(4), b = 0.83920(4), c = 1.65787(8) nm, Z = 2, and D = 2.041 g/cm(3). In this paper, the interaction of complex with Ct-DNA was investigated by UV-visible and viscosimetric techniques. Upon addition of the complex, important changes were observed in the characteristic UV-Vis bands (hyperchromism) of calf thymus DNA and some changes in specific viscosity. The experimental results showed that the complex is bound to DNA intercalative (intercalation binding).

以盐酸2-氨基乙硫醇和七水合硫酸锌为原料，在水溶液中合成了锌（II）配合物（C2H6NS）2Zn·ZnCl2。通过元素分析、红外光谱、单晶X射线衍射和热重分析对配合物的组成和结构进行了表征。锌（II）配合物的晶体结构属于单斜晶系，空间群为P 21/n，晶胞参数为a = 0.84294(4)，b = 0.83920(4)，c = 1.65787(8) nm，Z = 2，D = 2.041 g/cm³。本文采用紫外可见光谱和粘度测定技术研究了该配合物与小牛胸腺DNA（Ct-DNA）的相互作用。加入配合物后，观察到小牛胸腺DNA特征紫外可见吸收带发生了重要变化（增色效应），比浓粘度也有一些变化。实验结果表明，该配合物以插入方式与DNA结合（插入结合）。