Teng Fu-Yuan, Chen Wen-Cheng, Wang Yin-Lai, Hung Chun-Cheng, Tseng Chun-Chieh
Department of Dentistry, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan; School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Department of Prosthodontics, Kaohsiung Medical University Hospital, No. 100 Tzyou 1st Road, Kaohsiung 80708, Taiwan.
Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, College of Engineering, Feng Chia University, Taichung, Taiwan.
Bioinorg Chem Appl. 2016;2016:3837679. doi: 10.1155/2016/3837679. Epub 2016 Feb 8.
This study designed a biomimetic implant for reducing healing time and achieving early osseointegration to create an active surface. Bone morphogenetic protein-2 (BMP-2) is a strong regulator protein in osteogenic pathways. Due to hardly maintaining BMP-2 biological function and specificity, BMP-2 efficient delivery on implant surfaces is the main challenge for the clinic application. In this study, a novel method for synthesizing functionalized silane film for superior modification with BMP-2 on titanium surfaces is proposed. Three groups were compared with and without BMP-2 on modified titanium surfaces in vitro and in vivo: mechanical grinding; electrochemical modification through potentiostatic anodization (ECH); and sandblasting, alkali heating, and etching (SMART). Cell tests indicated that the ECH and SMART groups with BMP-2 markedly promoted D1 cell activity and differentiation compared with the groups without BMP-2. Moreover, the SMART group with a BMP-2 surface markedly promoted early alkaline phosphatase expression in the D1 cells compared with the other surface groups. Compared with these groups in vivo, SMART silaning with BMP-2 showed superior bone quality and created contact areas between implant and surrounding bones. The SMART group with BMP-2 could promote cell mineralization in vitro and osseointegration in vivo, indicating potential clinical use.
本研究设计了一种仿生植入物，以缩短愈合时间并实现早期骨整合，从而创建一个活性表面。骨形态发生蛋白-2（BMP-2）是成骨途径中的一种强效调节蛋白。由于难以维持BMP-2的生物学功能和特异性，在植入物表面高效递送BMP-2是临床应用的主要挑战。在本研究中，提出了一种在钛表面合成用于用BMP-2进行卓越改性的功能化硅烷膜的新方法。在体外和体内对改性钛表面上有无BMP-2的三组进行了比较：机械研磨；通过恒电位阳极氧化（ECH）进行电化学改性；以及喷砂、碱热和蚀刻（SMART）。细胞测试表明，与无BMP-2的组相比，有BMP-2的ECH组和SMART组显著促进了D1细胞的活性和分化。此外，与其他表面组相比，具有BMP-2表面的SMART组显著促进了D1细胞中早期碱性磷酸酶的表达。与体内的这些组相比，用BMP-2进行SMART硅烷化显示出优异的骨质量，并在植入物与周围骨骼之间形成了接触区域。具有BMP-2的SMART组可在体外促进细胞矿化，并在体内促进骨整合，表明其具有潜在的临床应用价值。
