Allina D O, Kekeeva T V, Moskvina L V, Shikeeva A A, Andreeva Yu Yu, Zavalishina L E, Frank G A
Russian Medical Academy of Postgraduate Education, Moscow.
Russian Medical Academy of Postgraduate Education, Moscow; Research Centre for Medical Genetics, Moscow.
Arkh Patol. 2015 Sep-Oct;77(5):36-42. doi: 10.17116/patol201577536-42.
to estimate the diagnostic and prognostic value of analyzing the abnormal overexpression of the chimeric protein ERG, encoded by the chimeric gene TMPRSS2/ERG, in prostatic neoplasias.
A total of 100 prostate adenocarcinoma samples were examined. The presence of tumor and high-grade prostatic intraepithelial neoplasia (hPIN) was verified by immunohistochemical tests using anti-P504S and anti-34βE12 antibodies in serial sections; RT-PCR was employed to analyze the chimeric transcript TMPRSS2/ERG in 30 prostate adenocarcinoma samples.
ERG expression was noted in 46% of the adenocarcinomas and in 21% of hPIN. Eight (8%) patients were observed to have heterogeneous ERG expression: the marked reaction in some tumor portions was concurrent with its complete absence in others. Furthermore, there was ERG expression in all cases of intraductal (noninvasive) carcinoma (the foci of intraductal carcinoma were assessed as atypical cribriform lesions by light microscopy). The prognostic value of ERG expression could not be determined at the current stage of the investigation.
The relatively low rate of ERG-positive hPIN counts in favor of the limited role of this marker in the differential diagnosis of hPIN. ERG in combination with P504S and 34βE12 is an informative marker for the differential diagnosis of hPIN with intraductal carcinoma.
评估分析由嵌合基因TMPRSS2/ERG编码的嵌合蛋白ERG异常过表达在前列腺肿瘤中的诊断和预后价值。
共检测100例前列腺腺癌样本。通过在连续切片中使用抗P504S和抗34βE12抗体的免疫组织化学试验来验证肿瘤和高级别前列腺上皮内瘤变（hPIN）的存在；采用逆转录聚合酶链反应（RT-PCR）分析30例前列腺腺癌样本中的嵌合转录本TMPRSS2/ERG。
在46%的腺癌和21%的hPIN中观察到ERG表达。8%（8例）患者存在ERG异质性表达：在一些肿瘤部分有明显反应，而在其他部分则完全没有。此外，在所有导管内（非侵袭性）癌病例中均有ERG表达（通过光学显微镜将导管内癌灶评估为非典型筛状病变）。在研究的现阶段，无法确定ERG表达的预后价值。
ERG阳性hPIN计数相对较低，表明该标志物在hPIN的鉴别诊断中作用有限。ERG与P504S和34βE12联合是鉴别hPIN与导管内癌的一个有价值的标志物。
