在嗜碱芽孢杆菌N16-5中鉴定出的一种新型甘露寡糖结合蛋白参与甘露聚糖利用。
A Novel Manno-Oligosaccharide Binding Protein Identified in Alkaliphilic Bacillus sp. N16-5 Is Involved in Mannan Utilization.
作者信息
Song Yajian, Li Jinshan, Meng Shan, Yin Liang, Xue Yanfen, Ma Yanhe
机构信息
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
出版信息
PLoS One. 2016 Mar 15;11(3):e0150059. doi: 10.1371/journal.pone.0150059. eCollection 2016.
ManH, a novel substrate-binding protein of an ABC transporter, was identified from the mannan utilization gene cluster of Bacillus sp. N16-5. We cloned, overexpressed, and purified ManH and measured its binding affinity to different substrates by isothermal titration calorimetry. ManH binds to mannotriose, mannotetraose, mannopentose, and galactosyl-mannotriose with dissociation constants in the micromolar range. Deletion of manH led to decreased growth ability of the strain when cultivated in medium with manno-oligosaccharides or mannan as the carbon source. ManH belongs to a manno-oligosaccharide transporter and plays an important role in mannan utilization by Bacillus sp. N16-5.
ManH是一种ABC转运蛋白的新型底物结合蛋白，从芽孢杆菌属N16 - 5的甘露聚糖利用基因簇中鉴定得到。我们克隆、过表达并纯化了ManH，并通过等温滴定量热法测量了它与不同底物的结合亲和力。ManH与甘露三糖、甘露四糖、甘露五糖和半乳糖基甘露三糖结合，解离常数在微摩尔范围内。缺失manH导致该菌株在以甘露寡糖或甘露聚糖作为碳源的培养基中培养时生长能力下降。ManH属于甘露寡糖转运蛋白，在芽孢杆菌属N16 - 5利用甘露聚糖过程中发挥重要作用。
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