Vieira Andres C, Cifuentes José Manuel, Bermúdez Roberto, Ferreiro Sara F, Castro Albina Román, Botana Luis M
Departamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo 27002, Spain.
Departamento de Anatomía, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo 27002, Spain.
Toxins (Basel). 2016 Mar 10;8(3):68. doi: 10.3390/toxins8030068.
Domoic acid (DA) is one of the best known marine toxins, causative of important neurotoxic alterations. DA effects are documented both in wildlife and experimental assays, showing that this toxin causes severe injuries principally in the hippocampal area. In the present study we have addressed the long-term toxicological effects (30 days) of DA intraperitoneal administration in rats. Different histological techniques were employed in order to study DA toxicity in heart, an organ which has not been thoroughly studied after DA intoxication to date. The presence of DA was detected by immunohistochemical assays, and cellular alterations were observed both by optical and transmission electron microscopy. Although histological staining methods did not provide any observable tissue damage, transmission electron microscopy showed several injuries: a moderate lysis of myofibrils and loss of mitochondrial conformation. This is the first time the association between heart damage and the presence of the toxin has been observed.
软骨藻酸（DA）是最著名的海洋毒素之一，可导致重要的神经毒性改变。DA对野生动物和实验检测的影响均有文献记载，表明这种毒素主要在海马区造成严重损伤。在本研究中，我们探讨了大鼠腹腔注射DA的长期毒理学效应（30天）。为了研究DA对心脏的毒性，采用了不同的组织学技术，心脏是迄今为止在DA中毒后尚未得到充分研究的器官。通过免疫组织化学检测法检测DA的存在，并通过光学显微镜和透射电子显微镜观察细胞变化。尽管组织学染色方法未显示任何可观察到的组织损伤，但透射电子显微镜显示出一些损伤：肌原纤维中度溶解和线粒体形态丧失。这是首次观察到心脏损伤与毒素存在之间的关联。
