Escobar Sebastián, Rocha Ana, Felip Alicia, Carrillo Manuel, Zanuy Silvia, Kah Olivier, Servili Arianna
Department of Fish Physiology and Biotechnology, Instituto de Acuicultura de Torre la Sal (CSIC), Ribera de Cabanes s/n, 12595 Torre la Sal, Castellón, Spain.
Department of Fish Physiology and Biotechnology, Instituto de Acuicultura de Torre la Sal (CSIC), Ribera de Cabanes s/n, 12595 Torre la Sal, Castellón, Spain.
Gen Comp Endocrinol. 2016 Apr 1;229:100-11. doi: 10.1016/j.ygcen.2016.03.017. Epub 2016 Mar 12.
In this study, we report the cloning of three transcripts for leptin receptor in the European sea bass, a marine teleost of economic interest. The two shortest variants, generated by different splice sites, encode all functional extracellular and intracellular domains but missed the transmembrane domain. The resulting proteins are therefore potential soluble binding proteins for leptin. The longest transcript (3605bp), termed sblepr, includes all the essential domains for binding and transduction of the signal. Thus, it is proposed as the ortholog for the human LEPR gene, the main responsible for leptin signaling. Phylogenetic analysis shows the sblepr clustered within the teleost leptin receptor group in 100% of the bootstrap replicates. The neuroanatomical localization of sblepr expressing cells has been assessed by in situ hybridization in brains of sea bass of both sexes during their first sexual maturation. At histological level, the distribution pattern of sblepr expressing cells in the brain shows no clear differences regarding sex or reproductive season. Transcripts of the sblepr have a widespread distribution throughout the forebrain and midbrain until the caudal portion of the hypothalamus. A high hybridization signal is detected in the telencephalon, preoptic area, medial basal and caudal hypothalamus and in the pituitary gland. In a more caudal region, sblepr expressing cells are identified in the longitudinal torus. The expression pattern observed for sblepr suggests that in sea bass, leptin is very likely to be involved in the control of food intake, energy reserves and reproduction.
在本研究中，我们报告了欧洲海鲈（一种具有经济价值的海洋硬骨鱼）中瘦素受体的三个转录本的克隆情况。由不同剪接位点产生的两个最短变体编码了所有功能性细胞外和细胞内结构域，但缺少跨膜结构域。因此，产生的蛋白质是瘦素的潜在可溶性结合蛋白。最长的转录本（3605bp），称为sblepr，包含了信号结合和转导的所有必需结构域。因此，它被认为是人类LEPR基因的直系同源物，是瘦素信号传导的主要负责者。系统发育分析表明，在100%的自展重复中，sblepr聚集在硬骨鱼瘦素受体组内。在性成熟初期，通过原位杂交对两性海鲈大脑中表达sblepr的细胞进行了神经解剖定位。在组织学水平上，大脑中表达sblepr的细胞的分布模式在性别或繁殖季节方面没有明显差异。sblepr的转录本在前脑和中脑广泛分布，一直到下丘脑的尾部。在端脑、视前区、内侧基底和尾侧下丘脑以及垂体中检测到高杂交信号。在更靠后的区域，在纵向视丘中鉴定出表达sblepr的细胞。观察到的sblepr表达模式表明，在海鲈中，瘦素很可能参与食物摄入、能量储备和繁殖的控制。
