Xiong Gordon M, Yap Yi Zhen, Choong Cleo
School of Materials Science & Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore.
KK Research Centre, KK Women's & Children Hospital, 100 Bukit Timah Road, 229899 Singapore.
Nanomedicine (Lond). 2016 Apr;11(7):749-65. doi: 10.2217/nnm.16.13. Epub 2016 Mar 16.
To perform one-pot synthesis of heparin-immobilized polypyrrole (PPy) nanoparticles and evaluate the use of these nanoparticles for the delivery of VEGF.
MATERIALS & METHODS: Heparin-stabilized synthesis of PPy nanoparticles was performed via oxidative polymerization. VEGF-bound PPy-heparin nanoparticles were delivered to endothelial cells and bioactivity of VEGF was assessed by Matrigel tube formation.
Size-controllable synthesis of heparin-doped PPy nanoparticles was achieved, and heparin promoted the conjugation of VEGF. Angiogenic activity of the VEGF-conjugated PPy nanoparticles was verified.
Heparin-doped PPy nanoparticles can be synthesized using one-pot reaction and provide a delivery platform by which VEGF can be conjugated onto.
进行肝素固定化聚吡咯（PPy）纳米颗粒的一锅法合成，并评估这些纳米颗粒用于递送血管内皮生长因子（VEGF）的用途。
通过氧化聚合进行肝素稳定的PPy纳米颗粒合成。将结合VEGF的PPy-肝素纳米颗粒递送至内皮细胞，并通过基质胶管形成评估VEGF的生物活性。
实现了肝素掺杂的PPy纳米颗粒的尺寸可控合成，且肝素促进了VEGF的结合。验证了VEGF共轭PPy纳米颗粒的血管生成活性。
肝素掺杂的PPy纳米颗粒可通过一锅法反应合成，并提供一个可将VEGF共轭到其上的递送平台。
