Cao Yue, Dong Ya-Xian, Xu Jie, Chu Guo-Liang, Yang Zhi-Hua, Liu Yan-Ming
Department of Medical Technology, Medical College of Shaoguan University, Shaoguan, Guangdong Province, China.
Department of Neurology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province, China.
Neural Regen Res. 2016 Jan;11(1):132-6. doi: 10.4103/1673-5374.175059.
NgR, the receptor for the neurite outgrowth inhibitor Nogo-66, plays a critical role in the plasticity and regeneration of the nervous system after injury such as ischemic stroke. In the present study, we used immunohistochemistry to investigate the regional expression of NgR in rat brain following middle cerebral artery occlusion (MCAO). NgR protein expression was not observed in the center of the lesion, but was elevated in the marginal zone compared with control and sham-operated rats. The cerebral cortex and hippocampus (CA1, CA2, and CA3) showed the greatest expression of NgR. Furthermore, NgR expression was higher in the ipsilesional hemisphere than on the control side in the same coronal section. Although time-dependent changes in NgR expression across brain regions had their own characteristics, the overall trend complied with the following rules: NgR expression changes with time showed two peaks and one trough; the first peak in expression appeared between 1 and 3 days after MCAO; expression declined at 5 days; and the second peak occurred at 28 days.
NgR是神经突生长抑制因子Nogo-66的受体，在诸如缺血性中风等损伤后的神经系统可塑性和再生中起关键作用。在本研究中，我们采用免疫组织化学方法研究大脑中动脉闭塞（MCAO）后大鼠脑内NgR的区域表达。在损伤中心未观察到NgR蛋白表达，但与对照组和假手术组大鼠相比，边缘区的NgR蛋白表达升高。大脑皮层和海马体（CA1、CA2和CA3）显示出最高的NgR表达。此外，在同一冠状切片中，患侧半球的NgR表达高于对照侧。尽管不同脑区NgR表达的时间依赖性变化有其自身特点，但总体趋势符合以下规律：NgR表达随时间变化呈现两个峰值和一个谷值；表达的第一个峰值出现在MCAO后第1至3天；在第5天表达下降；第二个峰值出现在第28天。
