Min You-Jiang, Yao Hai-Hua, Wang Li, Cheng Li-Hong, Hong En-Si
School of Traditional Chinese Medicine, Nanchang Medical College, Nanchang, 330052, Jiangxi, China.
Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, 330006, Jiangxi, China.
J Tradit Complement Med. 2023 Mar 22;13(5):430-440. doi: 10.1016/j.jtcme.2023.03.006. eCollection 2023 Sep.
In China, acupuncture and moxibustion have been used effectively to treat various diseases for thousands of years. However, the evidence for a difference in the efficacies of moxibustion and acupuncture in cerebral infarction treatment is scarce. We aimed to compare the effects of acupuncture and moxibustion treatment on the Nogo/NgR signaling pathway in rats with cerebral ischemia/reperfusion (I/R) injury.
Eighty male SD rats were randomly divided into five groups, based on treatment received: sham surgery (sham group), middle cerebral artery occlusion (MCAO, MCAO group), MCAO and NEP(1-40) inhibitor injection (MCAO + block group), MCAO and moxibustion (MCAO + moxi group), and MCAO and minimal acupuncture (MCAO + MA group). Neurological status was evaluated before treatment, and cerebral infarction volume (IV) and neurological function; Nogo-A, NgR, p75NTR, and LINGO-1 expressions; and NgR and LINGO-1 co-expression were assessed after treatment.
After treatment, barring Nogo-A mRNA and protein expression in the MCAO + block group, the Longa score and IV significantly decreased; Nogo-A, NgR, p75NTR, and LINGO-1 mRNA and protein expressions as well as NgR and LINGO-1 co-expression significantly decreased in cerebral tissues; whereas the BWT score increased (P < 0.01) in the MCAO + moxi group, compared with the MCAO group. Except for NgR and LINGO-1 protein expressions, there were no significant differences in the abovementioned parameters between rats that underwent acupuncture and moxibustion. Acupuncture and moxibustion have similar effects on Nogo/NgR signaling pathway inhibition after cerebral infarction.
在中国，针灸已被有效用于治疗各种疾病达数千年之久。然而，关于艾灸与针刺在脑梗死治疗中疗效差异的证据却很匮乏。我们旨在比较针刺与艾灸治疗对脑缺血/再灌注（I/R）损伤大鼠中Nogo/NgR信号通路的影响。
80只雄性SD大鼠根据接受的治疗随机分为五组：假手术组（假手术组）、大脑中动脉闭塞组（MCAO，MCAO组）、MCAO并注射NEP(1 - 40)抑制剂组（MCAO + 阻断组）、MCAO并艾灸组（MCAO + 艾灸组）以及MCAO并微针针刺组（MCAO + MA组）。治疗前评估神经状态，并在治疗后评估脑梗死体积（IV）和神经功能；Nogo - A、NgR、p75NTR和LINGO - 1表达；以及NgR和LINGO - 1共表达。
治疗后，除MCAO + 阻断组中Nogo - A mRNA和蛋白表达外：Longa评分和IV显著降低；脑组织中Nogo - A、NgR、p75NTR和LINGO - 1 mRNA及蛋白表达以及NgR和LINGO - 1共表达显著降低；而与MCAO组相比，MCAO + 艾灸组的BWT评分升高（P < 0.01）。除NgR和LINGO - 1蛋白表达外，针刺与艾灸大鼠上述参数无显著差异。针刺与艾灸在脑梗死发生后对Nogo/NgR信号通路抑制具有相似作用。
