Wang Xue-jun, Min Chang-li, Yang Yan
Zhong Yao Cai. 2015 Aug;38(8):1566-70.
To isolate and identify the biocontrol actinomycete from composting, and to investigate its potted control effect against Botrytis cinerea.
Actinomycetes were isolated from composting by pour plate method. Inhibitory activities of acti- nomycetes were determined by dilution plate method and cylinder plate method. Strain with high activities was identified based on mor- phology and biochemical characterization and 16S rDNA gene sequence analysis. And its potted control effect was also investigated.
31 strains of actinomycetes were isolated, three of them had inhibitory effects on the test strains. Of which, the strain DF02 showed antagonistic to five plant pathogenic fungi of all the tested strains, and its inhibitory effect on Botrytis cinerea was the strongest. The results of potted control effect test showed that the protective and therapeutic efficacy of the fermentation suspension to Botrytis cinerea was 58.47% and 53.83%, respectively. According to taxonomic identification, the strain DF02 was identified as Strepto- myces neopeptinius.
Strain DF02 is a biocontrol actinomycete with broad development potential, and these results provide ex- perimental basis for the biocontrol of Botrytis cinerea.
从堆肥中分离鉴定生防放线菌，并研究其对灰霉病的盆栽防治效果。
采用倾注平板法从堆肥中分离放线菌。采用稀释平板法和试管平板法测定放线菌的抑菌活性。根据形态学、生化特性及16S rDNA基因序列分析对高活性菌株进行鉴定，并研究其盆栽防治效果。
分离得到31株放线菌，其中3株对供试菌株有抑制作用。其中，菌株DF02对所有供试的5种植物病原真菌均表现出拮抗作用，且对灰霉病的抑制作用最强。盆栽防治效果试验结果表明，发酵液对灰霉病的保护和治疗效果分别为58.47%和53.83%。经分类鉴定，菌株DF02被鉴定为新肽链霉菌。
菌株DF02是一种具有广阔开发潜力的生防放线菌，研究结果为灰霉病的生物防治提供了实验依据。
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