Xia H, Li Y Y, С Liu Z, Li Y Q, Chen J
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai, 200240 P. R. China.
The key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, 200240 P. R. China.
Mol Biol (Mosk). 2018 Sep-Oct;52(5):773-781. doi: 10.1134/S0026898418050142.
Chitinases expressed by some beneficial fungi are crucial for the biocontrol of phytopathogens. The activity of chitinolytic strains of Trichoderma sp. may be enhanced by increasing the expression of chitinases. We describe the Trichoderma strain Mchit42 which expresses a transgenic chitinase chit42 from Metarhizium anisopliae. Inhibitory effects against plant pathogens were tested. Comparison of WT (T30) and OE (Mchit42) indicated that overexpression of M. anisopliae chit42 did not alter Trichoderma growth, while enhancing the expression of endogenous chitinase, β-l,3-glucanases, and polygalacturonase and increasing the antagonistic activity of Trichoderma against Botrytis cinerea. This work confirmed that the expression of the entomopathogenic fungi-sourced chit42 genes in Trichoderma harzianum enhances the efficiency of Trichoderma biocontrol against targeted pathogens.
一些有益真菌表达的几丁质酶对植物病原体的生物防治至关重要。木霉菌几丁质分解菌株的活性可通过增加几丁质酶的表达来增强。我们描述了木霉菌株Mchit42，它表达来自绿僵菌的转基因几丁质酶chit42。测试了其对植物病原体的抑制作用。野生型（T30）和过表达型（Mchit42）的比较表明，绿僵菌chit42的过表达不会改变木霉菌的生长，同时增强了内源性几丁质酶、β-1,3-葡聚糖酶和多聚半乳糖醛酸酶的表达，并提高了木霉菌对灰葡萄孢的拮抗活性。这项工作证实，在哈茨木霉菌中表达昆虫病原真菌来源的chit42基因可提高木霉菌对目标病原体的生物防治效率。
