Wan Jinlin, Dong Ziqing, Lei Chen, Lu Feng
Guangzhou, Guangdong, People's Republic of China.
From the Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University.
Plast Reconstr Surg. 2016 Jul;138(1):109-120. doi: 10.1097/PRS.0000000000002305.
The tissue-engineering chamber technique can generate large volumes of adipose tissue, which provides a potential solution for the complex reconstruction of large soft-tissue defects. However, major drawbacks of this technique are the foreign-body reaction and the volume limitation imposed by the chamber.
In this study, the authors developed a novel tissue-engineering method using a specially designed external suspension device that generates an optimized volume of adipose flap and avoids the implantation of foreign material. The rabbits were processed using two different tissue-engineering methods, the external suspension device technique and the traditional tissue-engineering chamber technique.
The adipose flaps generated by the external suspension device had a normal adipose tissue structure that was as good as that generated by the traditional tissue-engineering chamber, but the flap volume was much larger. The final volume of the engineered adipose flap grew between weeks 0 and 36 from 5.1 ml to 30.7 ml in the traditional tissue-engineering chamber group and to 80.5 ml in the external suspension device group. During the generation process, there were no marked differences between the two methods in terms of structural and cellular changes of the flap, except that the flaps in the traditional tissue-engineering chamber group had a thicker capsule at the early stage. In addition, the enlarged flaps generated by the external suspension device could be reshaped into specific shapes by the implant chamber.
This minimally invasive external suspension device technique can generate large-volume adipose flaps. Combined with a reshaping method, this technique should facilitate clinical application of adipose tissue engineering.
组织工程腔室技术可生成大量脂肪组织，为大型软组织缺损的复杂重建提供了一种潜在解决方案。然而，该技术的主要缺点是异物反应和腔室造成的体积限制。
在本研究中，作者开发了一种新型组织工程方法，使用专门设计的外部悬吊装置，该装置可生成优化体积的脂肪瓣并避免植入异物。对兔子采用两种不同的组织工程方法进行处理，即外部悬吊装置技术和传统的组织工程腔室技术。
外部悬吊装置生成的脂肪瓣具有正常的脂肪组织结构，与传统组织工程腔室生成的脂肪瓣一样好，但瓣体积要大得多。在传统组织工程腔室组中，工程化脂肪瓣的最终体积在0至36周之间从5.1毫升增长到30.7毫升，而在外部悬吊装置组中增长到80.5毫升。在生成过程中，两种方法在瓣的结构和细胞变化方面没有明显差异，只是传统组织工程腔室组的瓣在早期有较厚的包膜。此外，外部悬吊装置生成的增大的瓣可通过植入腔室重塑成特定形状。
这种微创外部悬吊装置技术可生成大体积脂肪瓣。结合重塑方法，该技术应有助于脂肪组织工程的临床应用。
