Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, Guang Zhou, Guang Dong, P.R. China.
Department of Plastic and Cosmetic Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, P.R. China.
J Tissue Eng Regen Med. 2018 Mar;12(3):e1549-e1558. doi: 10.1002/term.2580. Epub 2017 Dec 17.
Tissue engineering chamber (TEC) is a technique that could incubate up to 16 folds volume increase of a fat flap. But the mechanism in the silicone chamber was still unknown. The function of exudate in the chamber was noticed recently. We developed a special model called fluid drainage model (FDM) that consisted of a traditional TEC on the back and paired fat flaps without a chamber in the groins. Then we used a silicon tube to dynamically transfer the exudate from dorsal TEC to one of the paired inguinal fat flap and the other inguinal fat flap with a sham tube was set as control. At Week 4, the volume of drainage group reached 8.7 ± 2.3 ml, 576 ± 152% to its original volume whereas the growth ratio of control group was only 130 ± 39%. Similar volume change and histological change were observed within fat flap from TEC model and drainage group. The exudate in the TEC is a heterogeneous cocktail contains cytokines as well as cells. Intriguingly, transferred exudate in the TEC model sustain the ability to incubate large amount of adipose tissue remotely.
组织工程室（TEC）是一种可以将脂肪皮瓣的体积增加 16 倍的技术。但硅酮室中的机制仍不清楚。最近注意到室中的渗出物的功能。我们开发了一种特殊的模型，称为引流模型（FDM），它由背部的传统 TEC 和腹股沟无室的配对脂肪瓣组成。然后，我们使用硅管将渗出物从背部 TEC 动态转移到配对的腹股沟脂肪瓣之一，而另一个腹股沟脂肪瓣则设置为假管作为对照。在第 4 周，引流组的体积达到 8.7±2.3ml，是原始体积的 576±152%，而对照组的生长比例仅为 130±39%。在 TEC 模型和引流组中，脂肪瓣内观察到相似的体积变化和组织学变化。TEC 中的渗出物是一种异质的混合物，包含细胞因子和细胞。有趣的是，TEC 模型中转移的渗出物保持了远程孵育大量脂肪组织的能力。
