Heit W, Hellriegel K P, Heimpel H, Gross R
Klin Wochenschr. 1977 Sep 15;55(18):895-8. doi: 10.1007/BF01478824.
In patients with haemopoietic failure, the low incidence of cells in mitosis often prevents successful chromosome preparations. By performing liquid cultures of myeloid cells a high incidence of mitoses could be obtained even in those cases failing to provide mitoses when the direct preparation technique was used. The cytogenetic studies demonstrated that the mitoses achieved by culture techniques were aneuploid and, thus, there is evidence that they are of leukaemic origin. The combination of short-term liquid culture and chromosome analysis appears to be a diagnostic approach in selected cases of hemopoietic insufficiency.
在造血功能衰竭患者中，有丝分裂细胞的低发生率常常妨碍成功制备染色体。通过进行髓细胞的液体培养，即使在使用直接制备技术未能获得有丝分裂的病例中，也能获得高发生率的有丝分裂。细胞遗传学研究表明，通过培养技术获得的有丝分裂是非整倍体的，因此，有证据表明它们起源于白血病。短期液体培养和染色体分析相结合似乎是某些造血功能不全病例的一种诊断方法。
