Gorjikhah Fatemeh, Azizi Jalalian Farid, Salehi Roya, Panahi Yunes, Hasanzadeh Arash, Alizadeh Effat, Akbarzadeh Abolfazl, Davaran Soodabeh
a Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
b Department of Medical Nanotechnology, Faculty of Advanced Medical Science , Tabriz University of Medical Sciences , Tabriz , Iran.
Artif Cells Nanomed Biotechnol. 2017 May;45(3):432-440. doi: 10.3109/21691401.2016.1160915. Epub 2016 Mar 22.
Among all cancers that affect women, breast cancer has most mortality rate. It is essential to attain more safe and efficient anticancer drugs. Recent advances in medical nanotechnology and biotechnology have caused in novel improvements in breast and other cancer drug delivery. Methotrexate is an anticancer drug that prevents the dihydrofolate reductase enzyme, which inhibits in the formation of DNA, RNA and proteins which have poor water-solubility. For enhancing the solubility and stability of drugs in delivery systems, we used methotrexate-loaded PLGA- beta-cyclodextrin nanoparticles. The PLGA- beta-cyclodextrin nanoparticles were synthesized by a double emulsion method and characterized with FT-IR and SEM. T47D breast cancer cell lines were treated with equal concentrations of methotrexate-loaded PLGA- beta-cyclodextrin nanoparticles and free methotrexate. MTT assay confirmed that methotrexate-loaded PLGA- beta-cyclodextrin nanoparticles enhanced cytotoxicity and drug delivery in T47D breast cancer cells. These results indicate that encapsulated drugs could be effective in controlled drug release for a sustained period would serve the purpose for long-term treatment of many diseases such as breast cancer.
在所有影响女性的癌症中，乳腺癌的死亡率最高。获得更安全、有效的抗癌药物至关重要。医学纳米技术和生物技术的最新进展已为乳腺癌及其他癌症的药物递送带来了新的改进。甲氨蝶呤是一种抗癌药物，它能抑制二氢叶酸还原酶，进而抑制DNA、RNA和蛋白质的形成，而这些物质的水溶性较差。为提高药物在递送系统中的溶解度和稳定性，我们使用了载有甲氨蝶呤的聚乳酸-羟基乙酸共聚物-β-环糊精纳米颗粒。聚乳酸-羟基乙酸共聚物-β-环糊精纳米颗粒通过双乳化法合成，并通过傅里叶变换红外光谱仪（FT-IR）和扫描电子显微镜（SEM）进行表征。用等浓度的载有甲氨蝶呤的聚乳酸-羟基乙酸共聚物-β-环糊精纳米颗粒和游离甲氨蝶呤处理T47D乳腺癌细胞系。MTT法证实，载有甲氨蝶呤的聚乳酸-羟基乙酸共聚物-β-环糊精纳米颗粒增强了对T47D乳腺癌细胞的细胞毒性和药物递送。这些结果表明，包封药物在持续的控释中可能有效，这将有助于实现对乳腺癌等多种疾病的长期治疗。
