Anusha Subramanian Mohanakrishnan, Leelaram Santharam, Surianarayanan Mahadevan
Chemical Engineering Department, Central Leather Research Institute (CLRI), Adyar, Chennai, Tamil Nadu, India.
Appl Biochem Biotechnol. 2016 Jul;179(6):1041-59. doi: 10.1007/s12010-016-2049-0. Epub 2016 Mar 22.
Bioplastic production from microbial sources is an emerging area which provides opportunities even to convert the wastes into bioplastics. Poly (3-hydroxybutyric acid), commonly called as PHB, is a bioplastic, which is stored as intracellular cytoplasmic inclusions in microorganisms. The objectives of this study are to calorimetrically monitor the PHB production and evaluate the thermokinetic data in a bioreaction calorimeter (BioRC1e). Thus, a well-known PHB-producing bacteria Ralstonia eutropha was selected for batch process in a bioreaction calorimeter. The metabolic heat generated was found to be correlated with the biomass, substrate consumption, oxygen uptake rate (OUR), carbon dioxide evolution rate (CER) and PHB production. The OUR pattern explained the oxidative metabolism of the strain R. eutropha. The heat yields due to biomass and glucose consumption during PHB production were found to be 12.56 and 13.56 kJ/g, respectively. The oxycalorific value obtained for the PHB production was 443.80 kJ/mol of O2. The concentration of PHB obtained in BioRC1e was 4.33 g/L with a production rate of 0.09 g/L/h. The chemical structure of the extracted PHB by R. eutropha was confirmed using fourier transform infrared spectroscopy (FT-IR) and (1)H and (13)C nuclear magnetic resonance (NMR) analysis.
利用微生物生产生物塑料是一个新兴领域，它甚至为将废物转化为生物塑料提供了机会。聚（3-羟基丁酸），通常称为PHB，是一种生物塑料，作为细胞内的细胞质内含物储存在微生物中。本研究的目的是通过量热法监测PHB的生产，并在生物反应量热仪（BioRC1e）中评估热动力学数据。因此，选择了一种著名的产PHB细菌——真养产碱杆菌，在生物反应量热仪中进行分批培养。发现产生的代谢热与生物量、底物消耗、氧摄取率（OUR）、二氧化碳释放率（CER）和PHB产量相关。OUR模式解释了真养产碱杆菌菌株的氧化代谢。在PHB生产过程中，由于生物量和葡萄糖消耗产生的热产率分别为12.56和13.56 kJ/g。PHB生产的氧热值为443.80 kJ/mol O2。在BioRC1e中获得的PHB浓度为4.33 g/L，生产率为0.09 g/L/h。利用傅里叶变换红外光谱（FT-IR）以及（1）H和（13）C核磁共振（NMR）分析，确认了真养产碱杆菌提取的PHB的化学结构。
