School of Pharmacy, University of East Anglia, Norwich, UK.
IRCCS-Istituto di Ricerche Farmacologiche 'Mario Negri', Milan, Italy.
Nanomedicine (Lond). 2016 Mar;11(6):643-56. doi: 10.2217/nnm.15.219. Epub 2016 Mar 22.
With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index.
We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice.
The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.
随着越来越多的应用领域对纳米粒子（NPs）的需求增加，迫切需要有效地评估其潜在毒性。我们提出了一种纳米颗粒危害评估方案，该方案将哺乳动物细胞毒性数据与胚胎脊椎动物畸形评分相结合，以确定整体毒性指数。
我们观察到，在暴露于一系列 NPs 后，爪蟾表型评分与基于细胞的体外测定具有很强的相关性。在体外和爪蟾中均无毒性的磁铁核 NPs 进一步在小鼠中被证实为无毒。
这些结果突出了爪蟾胚胎分析作为 NPs 毒性评估快速筛选方法的潜力，这可用于纳米材料的常规测试。
