European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Nanobiosciences Unit, Ispra (VA), Italy.
Nanotoxicology. 2012 May;6(3):272-87. doi: 10.3109/17435390.2011.572302. Epub 2011 Apr 17.
Different in vitro assays are successfully used to determine the relative cytotoxicity of a broad range of compounds. Nevertheless, different research groups have pointed out the difficulty in using the same tests to assess the toxicity of nanoparticles (NPs). In this study, we evaluated the possible use of a microphysiometer, Bionas 2500 analyzing system Bionas GmbH®, to detect in real time changes in cell metabolisms linked to NPs exposure. We focused our work on response changes of fibroblast cultures linked to exposure by cobalt ferrite NPs and compared the results to conventional in vitro assays. The measurements with the microphysiometer showed a cobalt ferrite cytotoxic effect, confirmed by the Colony Forming Efficiency assay. In conclusion, this work demonstrated that the measurement of metabolic parameters with a microphysiometer is a promising method to assess the toxicity of NPs and offers the advantage to follow on-line the cell metabolic changes.
不同的体外检测方法被成功地用于确定广泛的化合物的相对细胞毒性。然而，不同的研究小组已经指出，使用相同的测试来评估纳米颗粒（NPs）的毒性存在困难。在本研究中，我们评估了微生理仪（Bionas 2500 分析系统，Bionas GmbH®）的可能用途，以实时检测与 NPs 暴露相关的细胞代谢变化。我们专注于与钴铁氧体 NPs 暴露相关的成纤维细胞培养物的反应变化，并将结果与传统的体外检测方法进行比较。微生理仪的测量显示钴铁氧体具有细胞毒性作用，这一结果得到集落形成效率检测的证实。总之，这项工作表明，使用微生理仪测量代谢参数是一种有前途的评估 NPs 毒性的方法，并且具有在线跟踪细胞代谢变化的优势。
