Felipe I, Bim S, Loyola W
Departamento de Patologia Geral, Universidade Estadual de Londrina, PR, Brasil.
Braz J Med Biol Res. 1989;22(10):1251-4.
We have evaluated the participation of mannose receptors on the surface of stimulated macrophages in the phagocytosis of Candida albicans in vitro. A dose-dependent 8.6 to 88.3% reduction of phagocytosis was observed in the presence of 0.5 to 5.0 mg/ml of the mannose-rich glycoprotein invertase (either native or denatured) in the incubation medium. Macrophages plated onto substrates coated with poly-L-lysine-mannan also showed a 99% reduction of phagocytic activity toward Candida albicans, but phagocytosis of IgG-coated erythrocytes was not inhibited under the same conditions. These results indicate that mannose receptors are involved in one of the initial steps of phagocytosis of Candida albicans by macrophages.
我们评估了刺激巨噬细胞表面的甘露糖受体在体外对白色念珠菌吞噬作用中的参与情况。在孵育培养基中存在0.5至5.0mg/ml富含甘露糖的糖蛋白转化酶（天然或变性）时，观察到吞噬作用呈剂量依赖性降低8.6%至88.3%。接种在涂有聚-L-赖氨酸-甘露聚糖的底物上的巨噬细胞对白色念珠菌的吞噬活性也降低了99%，但在相同条件下，IgG包被的红细胞的吞噬作用未受抑制。这些结果表明，甘露糖受体参与了巨噬细胞对白色念珠菌吞噬作用的初始步骤之一。
