Zhai Wanchen, Wei Enwei, Li Rui, Ji Tianyi, Jiang Yueyao, Wang Xiaoxiao, Liu Yiying, Ding Zhiying, Zhou Hongli
School of Pharmaceutical Sciences, Jilin University, Changchun 130012, PR China.
Bethune Institute of Epigenetic Medicine, The First Hospital, Jilin University, Changchun 130012, PR China.
ACS Omega. 2020 Dec 30;6(1):656-665. doi: 10.1021/acsomega.0c05171. eCollection 2021 Jan 12.
is widely used for stanching bleeding. In this study, polysaccharides from (BSP) were extracted by hot water. Four polysaccharides named BSP-1-BSP-4 were fractionated using DEAE-52 cellulose. BSP fractions contained sulfate, and the degrees of substitution of BSP-3 and BSP-4 were 1.59 and 1.70, respectively. Analysis of monosaccharide composition showed that four polysaccharides were mainly composed of mannan and glucose. The results showed that BSP-1-BSP-4 elicited pro-coagulant capacities by shortening the activating partial thromboplastin time, prothrombin time, and thrombin time and elevating the fibrinogen content. Immunomodulatory activity was evaluated by MTT assay, the pinocytic capacity and NO production. Although BSP fractions did not affect RAW 264.7 cell viability, they, especially BSP-2, enhanced the immunomodulatory activity by increasing the pinocytic capacity and NO production. Overall, BSP may be developed as a potential coagulant with immunomodulatory effects.
被广泛用于止血。在本研究中，从[具体来源]提取了多糖（BSP）。使用DEAE - 52纤维素对四种名为BSP - 1至BSP - 4的多糖进行了分级分离。BSP级分含有硫酸盐，BSP - 3和BSP - 4的取代度分别为1.59和1.70。单糖组成分析表明，四种多糖主要由甘露聚糖和葡萄糖组成。结果表明，BSP - 1至BSP - 4通过缩短活化部分凝血活酶时间、凝血酶原时间和凝血酶时间以及提高纤维蛋白原含量来引发促凝血能力。通过MTT法、吞噬能力和一氧化氮产生来评估免疫调节活性。虽然BSP级分不影响RAW 264.7细胞活力，但它们，尤其是BSP - 2，通过增加吞噬能力和一氧化氮产生来增强免疫调节活性。总体而言，BSP可能被开发成为一种具有免疫调节作用的潜在凝血剂。
