Barth Susanne, Jankowska Marta Jolanta, Hodkinson Trevor Roland, Vellani Tia, Klaas Manfred
Teagasc Crops Environment and Land Use Programme, Oak Park Research Centre, Carlow, Ireland.
Department of Botany, School of Natural Sciences, Trinity College Dublin, Dublin, Ireland.
BMC Res Notes. 2016 Mar 22;9:184. doi: 10.1186/s13104-016-1994-6.
Forty three microsatellite markers were developed for further genetic characterisation of a forage and biomass grass crop, for which genomic resources are currently scarce. The microsatellite markers were developed from a normalized EST-SSR library. All of the 43 markers gave a clear banding pattern on 3% Metaphor agarose gels. Eight selected SSR markers were tested in detail for polymorphism across eleven DNA samples of large geographic distribution across Europe. The new set of 43 SSR markers will help future research to characterise the genetic structure and diversity of Phalaris arundinacea, with a potential to further understand its invasive character in North American wetlands, as well as aid in breeding work for desired biomass and forage traits. P. arundinacea is particularly valued in the northern latitude as a crop with high biomass potential, even more so on marginal lands.
开发了43个微卫星标记，用于对一种饲料和生物质草作物进行进一步的遗传特征分析，目前该作物的基因组资源稀缺。这些微卫星标记是从一个标准化的EST-SSR文库中开发出来的。所有43个标记在3%的Metaphor琼脂糖凝胶上都呈现出清晰的条带模式。对8个选定的SSR标记进行了详细测试，以检测其在欧洲广泛地理分布的11个DNA样本中的多态性。新的43个SSR标记集将有助于未来的研究，以表征虉草的遗传结构和多样性，有可能进一步了解其在北美湿地的入侵特性，并有助于开展具有所需生物质和饲料性状的育种工作。虉草在北纬地区作为一种具有高生物质潜力的作物特别受重视，在边际土地上更是如此。
