Huang Cyoung-Huei, Hou Yu-Chen, Pai Man-Hui, Yeh Chiu-Li, Yeh Sung-Ling
1 School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan.
2 Master Program in Food Safety, College of Nutrition, Taipei Medical University, Taipei, Taiwan.
JPEN J Parenter Enteral Nutr. 2017 May;41(4):647-656. doi: 10.1177/0148607116638493. Epub 2016 Mar 22.
This study evaluated the effect of different dietary ω-6/ω-3 polyunsaturated fatty acid (PUFA) ratios on modulating helper T (Th) and regulatory T (Treg) lymphocytes in mice with dextran sulfate sodium (DSS)-induced colitis.
There were 3 control and 3 colitis groups. Mice were fed for 24 days with diets with soybean oil (S), a mixture of soybean oil and low fish oil content (LF), or high fish oil content (HF). The ratio of ω-6/ω-3 PUFA in the LF diet was 4:1, and that in the HF diet was 2:1. The control groups drank distilled water while colitis groups were provided 2% DSS in drinking water during days 15-19. All mice drank distilled water from days 20-24 for recovery and were sacrificed on day 25.
Colitis resulted in higher blood Th1, Th2, and Th17 and lower Treg percentages. Also, plasma haptoglobin and proinflammatory chemokines were elevated in colon lavage fluid. Colitic groups with fish oil had lower inflammatory mediators in the plasma and colon lavage fluid. Furthermore, the percentages of blood Th1, Th2, and Th17 cells were lower, whereas Treg cell percentages were higher than those in the soybean oil group. The colitis group with an ω-6/ω-3 PUFA ratio of 2:1 had more pronounced effects than the group with a ratio of 4:1.
Diets with an ω-6/ω-3 PUFA ratio of 2:1 or 4:1 regulate the Th/Treg balance and attenuate inflammatory mediator production in colitis. Compared with the ω-6/ω-3 PUFA ratio of 4:1, the ratio of 2:1 was more effective in reducing inflammatory reactions in DSS-induced colitis.
本研究评估了不同饮食中ω-6/ω-3多不饱和脂肪酸（PUFA）比例对葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠辅助性T（Th）淋巴细胞和调节性T（Treg）淋巴细胞的调节作用。
设有3个对照组和3个结肠炎组。给小鼠喂食含大豆油（S）、低鱼油含量大豆油混合物（LF）或高鱼油含量（HF）的饮食24天。LF饮食中ω-6/ω-3 PUFA的比例为4:1，HF饮食中该比例为2:1。对照组饮用蒸馏水，而结肠炎组在第15 - 19天饮用含2% DSS的水。所有小鼠在第20 - 24天饮用蒸馏水以恢复，并在第25天处死。
结肠炎导致血液中Th1、Th2和Th17水平升高，Treg百分比降低。此外，结肠灌洗液中血浆触珠蛋白和促炎趋化因子升高。含鱼油的结肠炎组血浆和结肠灌洗液中的炎症介质较低。此外，血液中Th1、Th2和Th17细胞的百分比低于大豆油组，而Treg细胞百分比高于大豆油组。ω-6/ω-3 PUFA比例为2:1的结肠炎组比比例为4:1的组效果更显著。
ω-6/ω-3 PUFA比例为2:1或4:1的饮食可调节Th/Treg平衡并减轻结肠炎中炎症介质的产生。与ω-6/ω-3 PUFA比例为4:1相比，2:1的比例在减轻DSS诱导的结肠炎炎症反应方面更有效。
