Institute of Agro-products Processing and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, P.O. Box 5109, Beijing 100193, China.
School of Applied Sciences, RMIT University, City Campus, Melbourne, VIC 3001, Australia.
Food Funct. 2016 Apr;7(4):2034-42. doi: 10.1039/c5fo01612f.
Nanoliposome loaded with peanut peptide fraction (PPF) prepared by high pressure microfluidization (HPM) treatment was investigated as well as its stability and bioavailability. PPF showed hydrophilicity character with a solubility of 97.50 ± 2.31 mg mL(-1) in aqueous solution. HPM treatment can prepare nanoliposome but decreased encapsulation efficiency (EE). A pressure of 120 MPa was the appropriate parameter where the particle size and EE of nanolipsome was 79.67 ± 1.85 nm and 65.12 ± 2.96%, respectively. Crude liposome and nanoliposome both showed good stability under different pH conditions, even at pH value of 2.0. Nanoliposome behaved better in vitro controlled release than crude liposome. Most important of all, nanoliposome had the highest angiotension converting enzyme (ACE) inhibitory activity after simulated gastrointestinal tract (GIT) digestion. Morphology of digested liposome proved that nanoliposome can keep relative integrity in structure although it suffered a lot of attack.
采用高压微射流（HPM）处理制备的负载花生肽（PPF）的纳米脂质体，对其稳定性和生物利用度进行了研究。PPF 具有亲水性，在水溶液中的溶解度为 97.50 ± 2.31mg/mL。HPM 处理可以制备纳米脂质体，但降低了包封效率（EE）。120MPa 的压力是合适的参数，纳米脂质体的粒径和 EE 分别为 79.67 ± 1.85nm 和 65.12 ± 2.96%。粗脂质体和纳米脂质体在不同 pH 条件下均表现出良好的稳定性，即使在 pH 值为 2.0 时也是如此。纳米脂质体在体外控制释放方面的表现优于粗脂质体。最重要的是，纳米脂质体在模拟胃肠道（GIT）消化后具有最高的血管紧张素转化酶（ACE）抑制活性。消化后的脂质体形态学证明，尽管纳米脂质体受到了很多攻击，但它仍能保持相对完整的结构。
