Gu Yi-zhu, Zhou Wen, Zhai Guang-xi
Qilu Hospital, Shandong University, Jinan 250012, China.
Zhong Yao Cai. 2007 Aug;30(8):970-3.
To prepare puerarin liposome and study its oral absorption in rat.
Liposome was prepared through the way of film dispersion-ultrasonic. Free puerarin in liposome suspension was separated from liposome through ultrafiltration, and then encapsulation ratio of liposome was determined. Micro-morphology of liposme particles was observed under electronic transmission microscope. Puerarin concentration in blood was determined by HPLC.
The encapsulation ratio of puerarin in liposome was 53%, and liposome particles were global or elliptical. The diameter range of liposome particies was from 50 nm to 300 nm. The relative availability of puerarin liposme suspension to puerarin solution was 168%.
Liposome as a drug carrier can enhance the oral absorption of puerarin in rat.
制备葛根素脂质体并研究其在大鼠体内的口服吸收情况。
采用薄膜分散-超声法制备脂质体。通过超滤将脂质体混悬液中的游离葛根素与脂质体分离，然后测定脂质体的包封率。在透射电子显微镜下观察脂质体颗粒的微观形态。采用高效液相色谱法测定血液中葛根素的浓度。
葛根素脂质体的包封率为53%，脂质体颗粒呈球形或椭圆形。脂质体颗粒的直径范围为50nm至300nm。葛根素脂质体混悬液相对于葛根素溶液的相对生物利用度为168%。
脂质体作为药物载体可提高葛根素在大鼠体内的口服吸收。
