Türedi Sibel, Hancı Hatice, Çolakoğlu Serdar, Kaya Haydar, Odacı Ersan
a Department of Histology and Embryology, Faculty of Medicine , Karadeniz Technical University , Trabzon ;
b Department of Anatomy, Faculty of Medicine , Düzce University , Düzce ;
Int J Radiat Biol. 2016 Jun;92(6):329-37. doi: 10.3109/09553002.2016.1152415. Epub 2016 Mar 23.
The effects on human health of electromagnetic field (EMF) have begun to be seriously questioned with the entry into daily life of devices establishing EMF, such as cell phones, wireless fidelity, and masts. Recent studies have reported that exposure to EMF, particularly during pregnancy, affects the developing embryo/fetus. The aim of this study was therefore to examine the effects of exposure to continuous 900-Megahertz (MHz) EMF applied in the prenatal period on ovarian follicle development and oocyte differentiation. Six pregnant Sprague Dawley rats were divided equally into a non-exposed control group (CNGr) and a group (EMFGr) exposed to continuous 900-MHz EMF for 1 h daily, at the same time every day, on days 13-21 of pregnancy. New groups were established from pups obtained from both groups after birth. One group consisting of female pups from CNGr rats was adopted as newborn CNGr (New-CNGr, n = 6), and another group consisting of female pups from EMFGr rats was adopted as newborn EMFGr (New-EMFGr, n = 6). No procedure was performed on New-CNGr or New-EMFGr rats. All rat pups were sacrificed on the postnatal 34th day, and their ovarian tissues were removed. Follicle count, histological injury scoring and morphological assessment with apoptotic index criteria were performed with sections obtained following routine histological tissue preparation. Follicle count results revealed a statistically significant decrease in primordial and tertiary follicle numbers in New-EMFGr compared to New-CNGr (p < 0.05), while atretic follicle numbers and apoptotic index levels increased significantly (p < 0.05). Histopathological examination revealed severe follicle degeneration, vasocongestion, a low level of increased stromal fibrotic tissue and cytoplasmic vacuolization in granulosa cell in New-EMFGr. Prenatal exposure to continuous 900-MHz EMF for 1 h each day from days 13-21 led to a decrease in ovarian follicle reservoirs in female rat pups at the beginning of the prepubertal period.
随着手机、无线保真技术和信号塔等产生电磁场（EMF）的设备进入日常生活，电磁场对人类健康的影响开始受到严重质疑。最近的研究报告称，暴露于电磁场中，尤其是在孕期，会影响发育中的胚胎/胎儿。因此，本研究的目的是检验孕期暴露于连续900兆赫兹（MHz）电磁场对卵巢卵泡发育和卵母细胞分化的影响。将6只怀孕的斯普拉格-道利大鼠平均分为未暴露对照组（CNGr）和在怀孕第13至21天每天同一时间暴露于连续900-MHz电磁场1小时的组（EMFGr）。两组出生后的幼崽分别组成新的组。一组由CNGr大鼠的雌性幼崽组成，作为新生CNGr（New-CNGr，n = 6），另一组由EMFGr大鼠的雌性幼崽组成，作为新生EMFGr（New-EMFGr，n = 6）。未对New-CNGr或New-EMFGr大鼠进行任何操作。所有大鼠幼崽在出生后第34天处死，并取出其卵巢组织。在常规组织学制片后获得的切片上进行卵泡计数、组织学损伤评分以及基于凋亡指数标准的形态学评估。卵泡计数结果显示，与New-CNGr相比，New-EMFGr中原始卵泡和三级卵泡数量在统计学上显著减少（p < 0.05），而闭锁卵泡数量和凋亡指数水平显著增加（p < 0.05）。组织病理学检查显示，New-EMFGr中存在严重的卵泡退化、血管充血、基质纤维化组织轻度增加以及颗粒细胞胞质空泡化。在怀孕第13至21天每天暴露于连续900-MHz电磁场中1小时，会导致青春期前雌性大鼠幼崽的卵巢卵泡储备减少。
