Department of Histology and Embryology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey.
Acta Histochem. 2020 Dec;122(8):151652. doi: 10.1016/j.acthis.2020.151652. Epub 2020 Nov 12.
Several studies have investigated the effects of the electromagnetic field (EMF) on the central nervous system. However, we encountered no studies of the effects of EMF applied in the prenatal period on the offspring vertebrae. The aim of this study is to investigate the effect of a 900 megahertz (MHz) EMF applied to rat dams in the prenatal period on the vertebrae of rat pups. Female Sprague Dawley rats weighing 180-250 g were used in the experiment. Rats identified as pregnant were divided into two groups, control (n = 3) and EMF (n = 3). No EMF was applied to the control group pregnant rats. EMF was applied to the EMF group rats for 1 h daily on an equal and standard basis on prenatal days 13-21. All newborn rat pups were divided into pup control (n = 6) (PC) and pup EMF (n = 6) (PEMF) groups, and no treatment was performed on either. All animals were decapitated on day 32, and the spinal cord in the upper thoracic region was harvested. Vertebral tissues were subjected to routine histological procedures. Histopathological examination revealed that PEMF group vertebral cartilage had been converted into bone tissue. Comparison of central canal diameter and area values between the PEMF group and the PC group revealed statistically significant increases in the PEMF group (p = 0.000 and p = 0.001, respectively). Statistical analysıs revealed no significant difference in mean body weights between the two groups (p > 0.530). Based on these findings, we think that 900 MHz EMF applied in the prenatal period affects the development of the vertebrae. This effect causes pathological changes in the rat pup vertebrae. These findings now raise the question of whether EMF also has an impact on neurological and neurosurgical diseases involving the vertebrae.
已有多项研究调查了电磁场（EMF）对中枢神经系统的影响。然而，我们尚未发现研究产前应用 EMF 对后代椎骨的影响。本研究旨在调查在产前阶段对孕鼠应用 900 兆赫（MHz） EMF 对幼鼠椎骨的影响。实验中使用了 180-250 克的雌性 Sprague Dawley 大鼠。将怀孕的大鼠分为两组，对照组（n = 3）和 EMF 组（n = 3）。对照组的孕鼠不接受 EMF 处理。EMF 组的孕鼠在妊娠第 13-21 天每天接受 1 小时的 EMF 处理，处理方式相同且标准。所有新生幼鼠分为幼鼠对照组（n = 6）（PC）和幼鼠 EMF 组（n = 6）（PEMF），两组均不进行任何处理。所有动物均在第 32 天断头，采集上胸段脊髓。对椎骨组织进行常规组织学处理。组织病理学检查显示 PEMF 组的软骨已转化为骨组织。PEMF 组与 PC 组的中央管直径和面积值比较显示，PEMF 组的这些值显著增加（p = 0.000 和 p = 0.001）。两组间平均体重无统计学差异（p > 0.530）。基于这些发现，我们认为产前应用 900 MHz EMF 会影响椎骨的发育。这种影响导致幼鼠椎骨发生病理变化。这些发现现在提出了一个问题，即 EMF 是否也会对涉及椎骨的神经和神经外科疾病产生影响。
