Boonsaeng V, Chansiri K, Vilasineekul P, Wilairat P, Panyim S
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1989 Dec;20(4):519-22.
A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.
开发了一种简单的程序，可将血样直接点样到尼龙膜滤器上，无需用链霉蛋白酶或蛋白酶K处理样品，随后与32P标记的DNA探针pUNK1-45杂交。该探针能特异性检测恶性疟原虫DNA，且不与人、诺氏疟原虫、查巴迪疟原虫或食蟹猴疟原虫的DNA发生交叉反应。该探针能灵敏地检测出20微升血液中0.001%的疟原虫血症。
